LOCUS       sequence-28716-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X1 Zeo/pTER shGFP #2 (733-1)
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(177..201)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(285..305)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(703..714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aagtcttgga ttgggatctt ataagttccc tatcagtgat agagatcccc gctggagtac
       61 aactacaact tcaagagagt tgtagttgta ctccagcttt ttggaaaagc ttggtaccga
      121 gctcggatcc actagtccag tgtggtggaa ttcgcggccg cactcgagat atctagaccc
      181 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct
      241 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
      301 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
      361 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
      421 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
      481 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
      541 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
      601 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
      661 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
      721 aacttncttc cgcggctgct gc
//
