LOCUS       sequence-287160-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3539
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(611..635)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             643..684
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga ccgatccgtc gctggggctg
       61 acagtcccca tggcgccgcc tctggccccg ctccctcccc gggacccaaa cggggcggga
      121 tccgagtgga gaaagcccgg ggccgtgagc ttcgccgacg tggccgtgta cttctcccgg
      181 gaggagtggg gctgcctgcg gcccgcgcag agggccctgt accgggacgt gatgcgggag
      241 acctacggcc acctgggcgc gctcggtgag agccccacct gcttgcctgg gccctgcgcc
      301 tccacaggcc ctgccgcgcc tctgggagct gcgtgtggag ttgggggccc cggggccggg
      361 caggcggcct cctcgcagcg tggggtttgc gttcttctcc cccaggagtc ggaggcagca
      421 agccggcgct catctcctgg gtggaggaga aggccgaact gtgggatccg gctgcccagg
      481 atccggaggt ggcgaagtgt ccgacagaag cggacccagc agattccaga aacaaggaag
      541 aggaaagaca aagggaaggg acgggagccc tggagaagcc cgaccctgtg gccgccgggt
      601 ctcctgggct gcccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac
      661 cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga aagtatttcg
      721 atttcttggc tttatatatc ttgtggaaag gacgaatgtt tatacccagt gggaccttg
//
