LOCUS       sequence-287166-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3541
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(704..728)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             736..777
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg tgaaatgctg ctccgccatt
       61 ggatgtgctt ctcgctgctt gccaaattcg aagttaaaag gactgacatt tcacgtattc
      121 cccacagatg aaaacatcaa aaggaaatgg gtattagcaa tgaaaagact tgatgtgaat
      181 gcagccggca tttgggagcc taaaaaagga gatgtgttgt gttcgaggca ctttaagaag
      241 acagattttg acagaagtgc tccaaatatt aaactgaaac ctggagtcat accttctatc
      301 tttgattctc catatcacct acaggggaaa agagaaaaac ttcattgtag aaaaaacttc
      361 accctcaaaa ccgttccagc cactaactac aatcaccatc ttgttggtgc ttcctcatgt
      421 attgaagaat tccaatccca gttcattttt gaacatagct acagtgtaat ggacagtcca
      481 aagaaactta agcataaatt agatcatgtg atcggcgagc tagaggatac aaaggaaagt
      541 ctacggaatg ttttagaccg agaaaaacgt tttcagaaat cattgaggaa gacaatcagg
      601 gaattaaagg atgaatgtct gatcagccaa gaaacagcaa atagactgga cactttctgt
      661 tgggactgtt gtcaggagag catagaacag gactatattt catgcccaac tttcttgtac
      721 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
      781 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      841 aaggacgact ttgaggatat aggcccgcgg cc
//
