LOCUS       sequence-287169-         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3542
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(416..440)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             448..489
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gactgttgac attcagggat
       61 gtggccatag aattctctcg ggaggagtgg gaacacctgg actcagatca gaagctttta
      121 tatggggatg tgatgttaga gaactacgga aacctggtct ctctgggtct cgctgtctct
      181 aagccggacc tgatcacctt tttggagcaa aggaaagagc cctggaatgt gaagagtgca
      241 gagacagtag ccatccagcc agatatcttt tctcatgata ctcaaggcct cttaagaaag
      301 aagcttatag aagcatcatt ccaaaaagtg atattggatg gatatgggag ctgtggccct
      361 cagaatttaa acttaaggaa agagtgggaa agtgagggca aaataatcct atggtgccca
      421 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
      481 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
      541 atatcttgtg gaaaggacga tgccggggta cgcactagcc gata
//
