LOCUS       sequence-28717-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti X2 Hygro/pTER shATM #2 (w639-1)
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..42
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(173..197)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttngattgg gaatcttata agttccctat cagtgataga gatccctgaa gatggtgctc
       61 ataaattcaa gagatttatg agcaccatct tcatttttgg aaaagcttgg taccgagctc
      121 ggatccacta gtccagtgtg gtggaattcg cggccgcact cgagatatct agacccagct
      181 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agcccttcca
      241 gtcccccctt ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc
      301 ttaaaggtac cgggccctcg acggatctgc acccaatcgg caggcacggg cggcgatctc
      361 caatctgcgg gatcagtcag atcacccgag tgcgtgggca tgacaatcgt gccctgggga
      421 ccaacacaat ccagaagggc ctgaatcact gcgaccggcc ctcccgcgac ccagccgagc
      481 gagcttagcg aactgtggac gagaactgtg ccaccaagcg taaggccgtt ctctcgcatt
      541 tgccttgcta ggctcgcgcg agttgctggc tgaggcgttc tcgaaatcag ctcttgttcg
      601 gtcggcatct actctattcc tttgccctcg gacgagtgct gggggcgtcg gtttccacta
      661 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
      721 gtacgccgac agtcccggct ccggatcgga cgatgcgtcn catcgaccct gcgccaagct
      781 gct
//
