LOCUS       sequence-287172-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3543
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(497..521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             529..570
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga cttcagagtc gacgtcaccc
       61 ccagttgtgc ccccgctcca ctcccccaag tccccagtct ggcccacctt ccccttccac
      121 agggagggca gcagggtctg ggagcggggt ggtgtcccac ctcgggacct acccagccct
      181 ctgcccacca agcggaccag gacctattca gcgacagccc gggcctcagc tggccccgtg
      241 ttcaagggcg tctgtaagca gttctcacgc tcacagggcc atggcttcat cacccccgag
      301 aacgggtccg aggacatctt cgtacatgtg tctgacatcg agggggagta cgtgccagtg
      361 gagggcgacg aggtgaccta caagatgtgc cctatccctc ccaagaacca gaagttccag
      421 gccgtggagg tggtgctcac tcagctggcc ccccacactc cccacgagac gtggtctggc
      481 caggtcgtgg gctcctaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      541 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
      601 atttcgattt cttggcttta tatatcttgt ggaaaggacg acccaatact acgttttatt
      661 gcgat
//
