LOCUS       sequence-287175-        1361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3544
FEATURES             Location/Qualifiers
     source          1..1361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1193..1217)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1225..1266
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga gcaagccagc cggatcaaca
       61 agccgcattt tagatatccc ctgcaaagtg tgtggcgacc gcagctcggg gaagcactac
      121 ggggtctacg cctgcgacgg ctgctcaggt tttttcaaac ggagcatccg aaggaatagg
      181 acctatgtct gcaaatctgg aaaccaggga ggctgtccgg tggacaagac gcacagaaac
      241 cagtgcaggg cgtgtcggct gaagaagtgt ttggaagtca acatgaacaa agacgccgtg
      301 cagcacgagc gggggcctcg gacgtccacc atccgcaagc aagtggccct ctacttccgt
      361 ggacacaagg aggagaacgg ggccgccgcg cactttccct cggcggcgct ccctgcgccg
      421 gccttcttca ccgcggtcac gcagctggag ccgcacggcc tggagctggc cgcggtgtcc
      481 accactccag agcggcagac cctcgtgagc ctggctcagc ccacgcccaa gtacccccat
      541 gaagtgaatg ggaccccaat gtatctctat gaagtggcca cggagtcggt gtgtgaatca
      601 gctgccagac ttctcttcat gagcatcaag tgggctaaga gtgtgccagc cttctccacg
      661 ctgtctttgc aagaccagct gatgcttttg gaagatgctt ggagagaact gtttgttcta
      721 ggaatagcac aatgggccat tccggttgat gctaacactc tactggctgt atctggcatg
      781 aacggtgaca acacagattc ccagaagctg aacaagatca tatctgaaat acaggcttta
      841 caagaggtgg tggctcgatt tagacaactc cggttagatg ctactgaatt tgcctgtcta
      901 aaatgcatcg tcactttcaa agccgttcct acacatagtg gttctgaact gagaagtttc
      961 cggaatgctg ccgccattgc agcccttcaa gatgaggctc agctaacgct caacagctac
     1021 atccatacca gatatcccac tcaaccctgt cgctttggaa aactcctgtt gcttttgcca
     1081 gctttacgtt ctattagccc atcaactata gaagaagtgt ttttcaaaaa aaccatcggc
     1141 aatgtgccaa ttacaagact gctttcagat atgtacaaat ccagtgatat ctacccaact
     1201 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     1261 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1321 tcttgtggaa aggacgataa cccgggtcga caggctacgg g
//
