LOCUS       sequence-287178-        2162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3545
FEATURES             Location/Qualifiers
     source          1..2162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1994..2018)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2026..2067
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcggacag tgcggaacta
       61 aagcaaatgg ttatgagcct tagagtttct gaactccaag tactgttggg ctacgccggg
      121 agaaacaagc acggacgcaa acacgaactt ctcacaaaag ccctgcattt gctaaaggct
      181 ggctgtagtc ctgctgtgca aatgaaaatt aaggaactct ataggcggcg gttcccacag
      241 aaaatcatga cgcctgcaga cttgtccatc cccaacgtac attcaagtcc tatgccagca
      301 actttgtctc catctaccat tccacaactc acttacgatg gtcaccctgc atcatcgcca
      361 ttactccctg tttctcttct gggacctaaa catgaactgg aactcccaca tcttacatca
      421 gctcttcacc cagtccatcc ggatataaaa cttcaaaaat taccatttta tgatttactg
      481 gatgaactga taaaacccac cagtctagca tcagacaaca gtcagcgctt tcgagaaacc
      541 tgttttgcat ttgccttgac accacaacaa gtgcagcaaa tcagtagttc catggatatt
      601 tctgggacca aatgtgactt cacagtacag gtccagttaa ggttttgttt atcagaaacc
      661 agttgtccac aagaagatca cttcccaccc aatctttgtg tgaaagtgaa tacaaaacct
      721 tgcagccttc caggttacct tccacctaca aaaaatggcg tggaaccaaa gcgacccagc
      781 cgaccaatta atatcacctc acttgtccga ctgtccacaa cagtaccaaa cacgattgtt
      841 gtttcttgga ctgcagaaat tggaagaaac tattccatgg cagtatatct tgtaaaacag
      901 ttgtcctcaa cagttcttct tcagaggtta cgagcaaagg gaataaggaa tccggatcat
      961 tctagagctt taattaaaga gaagttgact gcggatccag acagtgaaat agctacaacc
     1021 agcctaaggg tttctctact atgtccactt ggtaaaatgc ggctgacaat tccgtgtcgg
     1081 gcccttacat gttctcatct acaatgtttt gacgcaactc tttacattca gatgaatgag
     1141 aaaaaaccaa cctgggtttg tcctgtctgt gataagaagg ctccatatga acaccttatt
     1201 attgatggct tgtttatgga aatcctaaag tactgtacag actgtgatga aatacaattt
     1261 aaggaggatg gcacttgggc accgatgaga tcaaaaaagg aagtacagga agtttctgcc
     1321 tcttacaatg gagtcgatgg atgcttgagc tccacattgg agcatcaggt agcgtctcac
     1381 caccagtcct caaataaaaa caagaaagta gaagtgattg acctaaccat agacagttca
     1441 tctgatgaag aggaagaaga gccatctgcc aagaggacct gtccttccct atctcccaca
     1501 tcaccactaa ataataaagg cattttaagt cttccacatc aagcatctcc agtatcccgc
     1561 accccaagcc ttcctgctgt agacacaagc tacattaata cctccctcat ccaagactat
     1621 aggcatcctt tccacatgac acccatgcct tacgacttac aaggattaga tttctttcct
     1681 ttcttatcag gagacaatca gcattacaac acctccttgc ttgccgctgc agcagcagca
     1741 gtttcagatg atcaagacct cctacactcg tctcggtttt tcccgtatac ctcctcacag
     1801 atgtttcttg atcagttaag tgcaggaggc agtacttctc tgccaaccac caatggaagc
     1861 agtagtggca gtaacagcag cctggtttct tccaacagcc taagggaaag ccatagccac
     1921 accgtcacaa acaggagcag cacggacacg gcatccatct ttggcatcat accagacatt
     1981 atttcattgg acttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     2041 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     2101 tcgatttctt ggctttatat atcttgtgga aaggacgaat caaatctgac tgtcacggca
     2161 gc
//
