LOCUS       sequence-287187-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3548
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(809..833)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             841..882
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cggcccgttg tgtggccgcc
       61 cactgcggca acaccaccaa gtctgggaag tcgctgttcc gctttcccaa ggaccgggcc
      121 gtgcggctgc tctgggaccg cttcgtgcgg ggttgccgcg ccgactggta cggaggcaat
      181 gaccgctcgg tcatctgctc tgaccacttt gccccagcct gttttgacgt ctcttcggtt
      241 atccagaaga acctgcgctt ctcccagcgc ctgaggctgg tggcaggcgc cgtgcccacc
      301 ctgcaccggg tgcccgcccc ggcacctaag aggggagagg agggagacca agcaggccgc
      361 ctggacacgc gaggagagct ccaggcagcc aggcattctg aggctgcccc aggtccagtc
      421 tcctgtacac gcccccgagc tgggaagcag gctgcagctt cacagattac gtgtgaaaat
      481 gaacttgtgc aaacccaacc ccatgctgat aatccatcta atactgtcac ttcagtacct
      541 actcactgtg aagaaggccc agtgcataaa agtacacaaa tttctttgaa aaggccccgt
      601 caccgtagtg tgggtattca agccaaagtg aaagcgtttg gaaaaagact gtgtaatgca
      661 actactcaga cagaggaatt gtggtctaga acttcctctc tctttgacat ttactccagt
      721 gattcagaaa cagatacaga ctgggatatc aagagtgaac agagtgattt gtcttatatg
      781 gctgtacagg tgaaagaaga aacatgttac ccaactttct tgtacaaagt ggttgatatc
      841 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
      901 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacttctat
      961 cagcctatct ctggggc
//
