LOCUS       sequence-287223-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP10 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP10
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(517..616)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(633..652)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(634..652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(657..673)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(657..673)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagttg gcatggctgg taacgctact gaggtgcctg
      121 ctaacagcac cgtgctgtcc ttctgcgcct tcgctgtgga cgccgctaag gcctacaagg
      181 actacctggc ttccggcgga cagcctatca ccaactgcgt gaagatgctg tgcacccaca
      241 ccggaaccgg tcaggccatc accgtgaccc cagaggctaa catggaccag gaatctttcg
      301 gtggcgccag ctgctgcctg tactgcagat gccacatcga ccaccccaac cctaagggtt
      361 tctgcgacct gaagggcaag tacgtgcaga tcccaaccac ctgcgctaac gaccccgtgg
      421 gattcaccct gaagaacacc gtgtgcaccg tgtgcggaat gtggaagggc tacggatgct
      481 cttgcgacca gctgagggaa cctatgctgc agtaataccc aactttcttg tacaaagttg
      541 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      601 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
      661 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
      721 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
      781 tgct
//
