LOCUS       sequence-287224-         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP10 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP10
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(522..621)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(637..654)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(637..653)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agttgggtat tactgcagca taggttccct
      121 cagctggtcg caagagcatc cgtagccctt ccacattccg cacacggtgc acacggtgtt
      181 cttcagggtg aatcccacgg ggtcgttagc gcaggtggtt gggatctgca cgtacttgcc
      241 cttcaggtcg cagaaaccct tagggttggg gtggtcgatg tggcatctgc agtacaggca
      301 gcagctggcg ccaccgaaag attcctggtc catgttagcc tctggggtca cggtgatggc
      361 ctgaccggtt ccggtgtggg tgcacagcat cttcacgcag ttggtgatag gctgtccgcc
      421 ggaagccagg tagtccttgt aggccttagc ggcgtccaca gcgaaggcgc agaaggacag
      481 cacggtgctg ttagcaggca cctcagtagc gttaccagcc atgccaactt ttttgtacaa
      541 agttggcatt ataaaaaagc attgctcatc aatttgttgc aacgaacagg tcactatcag
      601 tcaaaataaa atcattattt ggggcccgag cttaagactg gccgtcgttt tacaacgtcg
      661 tgactgg
//
