LOCUS       sequence-287293-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient 
            expression and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             709..732
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             769..792
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atttccggtg aattcgccgc caccatggcc gactcaaatg ggaccattac tgtagaggag
       61 ctgaaaaaac tgttggagca atggaacctt gtgattggat ttctcttctt gacatggatt
      121 tgtctcctgc aattcgcata cgcgaatcgc aaccgattcc tctatattat aaaacttata
      181 ttcctttggc tcctgtggcc tgtgaccctt gcctgcttcg tactcgcggc ggtgtatcga
      241 ataaactgga taactggcgg catagcaata gctatggcat gcctggtagg tttgatgtgg
      301 ctctcctatt ttatagcgtc attcagactt tttgcacgca cgcgaagcat gtggtcattt
      361 aaccctgaaa caaatatctt gcttaatgtc cccctgcatg gcacaatttt gacccgaccg
      421 ctccttgaat cagaattggt gattggagcc gtgatcctga ggggccactt gaggatcgca
      481 ggacatcatc ttggtcgctg tgatattaag gatttgccaa aagaaataac tgtagctacc
      541 agtcgaactt tgagctatta caagcttgga gcgtcccaaa gggtcgctgg cgatagtgga
      601 tttgcagcct attcacgcta caggataggg aactataaac tgaatacaga ccactcatca
      661 tcttctgata acattgcttt gctcgtccag ctcgaaggcg gcgggggatg gagccatcca
      721 caattcgaga aaggcggtgg ttcaggagga ggtagcgggg gtggatcatg gtcacatccg
      781 cagtttgaaa agtaaggatc ccgcccctct ccctcccccc ccc
//
