LOCUS       sequence-287298-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atttccggtg aattcgccgc caccatgaag attgtaaaca attggcttaa acaacttatt
       61 aaagtaaccc tggtattctt gtttgtcgcc gcaatctttt acttgataac accggtccac
      121 gtgatgtcta aacacacaga ctttagtagt gagataatcg gatataaagc tatcgatggc
      181 ggcgttaccc gcgacatcgc ttcaaccgac acatgtttcg ctaataagca cgccgatttt
      241 gacacttggt ttagtcaaag aggaggaagc tacacaaacg acaaggcttg tccgcttatc
      301 gcggctgtaa taacaaggga agttgggttt gtcgtgcctg gtttgccagg tactatactc
      361 agaaccacaa acggtgactt tctgcacttc ttgccaaggg tttttagcgc ggtaggcaat
      421 atctgctata cgccaagcaa gctgattgaa tacaccgact ttgcgacgag cgcatgtgtc
      481 ctcgctgcag agtgtacgat atttaaggat gcgagcggaa agcccgtacc ttactgctat
      541 gatactaatg tccttgaggg atcagttgcg tatgagagtc ttaggccgga tacacgatat
      601 gtgctcatgg atggttccat aatccagttc ccaaatacct acctcgaagg gtccgttcgg
      661 gtcgtcacca catttgatag cgaatattgc agacacggaa catgcgaacg gagtgaagca
      721 ggggtctgcg tctccacaag tgggcggtgg gtcctcaata acgattatta ccggagcctg
      781 cccggcgtgt tttgcggggt cgatgcggtt aacttgttga cgaatatgtt tacgcctctc
      841 atacaaccca ttggcgcact ggatatatct gcaagtatag tcgctggagg
//
