LOCUS       sequence-287311-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; 
            transient expression and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atttccggtg aattcgccgc caccatgagc gataacggcc cccaaaacca acggaatgcc
       61 cctcggataa catttggtgg ccctagtgat tccacaggaa gtaaccaaaa cggagaacgg
      121 tctggagccc gctctaaaca gaggcgacca cagggattgc caaataacac agcgtcatgg
      181 tttacagccc tgactcaaca tggaaaggag gacttgaaat ttccacgagg acagggtgta
      241 ccaataaaca caaacagtag tccagacgat cagatcgggt attatagaag ggccacgaga
      301 cgcatcagag gtggagatgg taaaatgaaa gatctcagtc cgcgctggta cttttattat
      361 ttgggaaccg gcccagaagc gggcctccca tatggagcca ataaggacgg gattatatgg
      421 gtggctacag agggagccct caatacgcca aaagatcaca tcggaacaag aaaccctgct
      481 aataatgccg caatcgtgct gcagttgcct caggggacga cgcttcctaa aggcttttac
      541 gcagaaggat cacgcggcgg cagccaagca tcctcaaggt ctagttccag aagtcgaaac
      601 agctccagaa actccacacc agggtccagt aggggcacaa gtccggcgcg gatggcgggc
      661 aacggcggag acgccgcact cgctctgttg ctcctggacc ggctcaacca acttgaatcc
      721 aagatgagtg gtaagggaca gcagcaacaa ggtcaaaccg taaccaagaa aagcgctgca
      781 gaagcttcta aaaaacctcg acaaaaacgg accgctacga aggcatataa cgttactcaa
      841 gccttcggga gacgagggcc ggagcaaacc caggggaatt ttggagatca ggagctcatc
      901 cggcaaggga cagattacaa acattggcct caaatcgctc agttcg
//
