LOCUS       sequence-287322-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttccggtga attcgccgcc accatgtcag cagacgcaca aagttttctg aaccgggtat
       61 gcggagtcag tgcggcgagg cttacgccct gtggtacggg aacatcaacg gacgttgttt
      121 accgcgcctt cgacatatac aatgataaag tcgccggctt cgcaaagttc cttaaaacga
      181 attgctgtag gttccaagaa aaggacgagg atgataattt gatagactcc tacttcgtgg
      241 tcaaacgaca cacgttttca aattatcagc acgaggaaac gatatataat cttcttaaag
      301 actgccctgc agtggcaaaa cacgacttct ttaagttccg cattgacggg gatatggtcc
      361 cccatatctc ccgacagaga ttgacgaaat atacaatggc ggatctggtg tacgccctga
      421 gacacttcga tgagggcaat tgtgatacac tcaaggagat tctcgtgacc tacaattgtt
      481 gcgatgatga ctatttcaat aagaaagact ggtacgactt cgtcgaaaac cctgatatat
      541 tgagggtgta cgcaaacctg ggtgaacgag taagacaggc acttctgaaa accgtccaat
      601 tctgcgacgc tatgagaaac gcgggcattg ttggtgtgct tacgctggat aaccaagacc
      661 tcaatggtaa ttggtacgat tttggggact tcatccaaac cactcctggc tcaggcgttc
      721 ccgtggtaga cagctactat tctttgttga tgccaattct tacactcaca agggccctga
      781 ccgctgagtc acacgtcgat acggacttga ccaagcccta catcaagtgg gatcttctga
      841 agtacgactt taccgaggaa cgattgaagc tttttgatcg ctactttaaa tactgggatc
      901 agacgtacca cccaaactgc gtgaattgcc tgg
//
