LOCUS       sequence-287328-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             869..892
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             929..952
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 tatttccggt gaattcgccg ccaccatgga cctttttatg agaatcttta ccatcgggac
       61 tgtcacactg aaacagggag aaattaagga cgcgaccccg agtgactttg tgagggccac
      121 tgctacaatt cccatccaag cctctcttcc attcggctgg ctcatagtgg gtgtggcact
      181 gctcgcggta ttccagtcag cgtctaagat cataacgctg aagaagagat ggcagttggc
      241 tctcagcaaa ggtgttcatt tcgtctgtaa cctgcttctt cttttcgtca ctgtctattc
      301 tcacttgctc ttggttgcag ccgggctgga agccccgttt ctgtatctgt acgcgctcgt
      361 ttacttcctc cagagcataa atttcgtacg aattattatg agactctggt tgtgctggaa
      421 gtgtcggagc aagaatcctc tcttgtatga cgccaattac ttcctttgtt ggcacactaa
      481 ttgttatgat tactgcatac cttacaatag cgttacctca tccatagtaa tcacctccgg
      541 tgacggaaca acgtcaccaa tctccgagca tgattaccag attggaggct atacggaaaa
      601 atgggagagt ggtgtcaagg actgtgtggt gctgcatagc tatttcacaa gcgattatta
      661 tcaactgtac agtacacagt tgtccacgga taccggggtc gagcatgtca cattttttat
      721 ttataacaag atcgttgatg agccagagga gcatgtgcag atccacacga tagatggaag
      781 ctccggtgta gtgaatccag tcatggaacc gatctatgac gagcccacca cgaccacatc
      841 tgtgcccctg ctcgaaggcg gcgggggatg gagccatcca caattcgaga aaggcggtgg
      901 ttcaggagga ggtagcgggg gtggatcatg gtcacatccg cagtttgaaa agtaaggatc
      961 ccgcccctct ccctcccccc ccc
//
