LOCUS       sequence-287340-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atttccggtg aattcgccgc caccatgtct ggttttagga aaatggcgtt ccccagcggt
       61 aaagttgaag gatgtatggt ccaagtaacc tgtggtacca ctacccttaa tgggctttgg
      121 ttggacgacg tagtctactg cccccgacac gtaatctgca ctagtgagga tatgcttaat
      181 cccaattacg aagacctttt gattcggaaa tccaatcaca acttcctggt ccaagcgggc
      241 aacgtccaac tcagggttat tggacatagt atgcagaatt gcgtactgaa gctcaaagtc
      301 gatactgcaa accccaagac gcccaagtat aaattcgtcc gaatccaacc aggccaaaca
      361 ttttccgtat tggcttgcta taatggaagc cccagcggtg tctaccaatg tgcaatgaga
      421 ccaaacttta cgataaaggg ttcatttctg aacggctctt gcggttccgt tggttttaac
      481 atcgactatg actgtgtatc cttttgctac atgcaccata tggaactccc taccggtgtc
      541 cacgccggta cagatctgga aggaaatttc tacggtccgt tcgttgaccg gcaaaccgcg
      601 caagcggctg gaaccgacac aacgattaca gtgaatgtgc tcgcgtggct gtacgcagca
      661 gtcataaacg gagacaggtg gtttctgaac cgatttacga cgactctcaa tgacttc
//
