LOCUS       sequence-28737-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Yeung T, Gilbert GE, Shi J, Silvius J, Kapus A, Grinstein S
  TITLE     Membrane phosphatidylserine regulates surface charge and protein 
            localization.
  JOURNAL   Science. 2008 Jan 11. 319(5860):210-3.
  PUBMED    18187657
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jan 11. 319(5860):210-3."
COMMENT     Sequence Label: Lact-C2-GFP-p416
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(10..12,13..15,16..726)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNHNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGIALGMDELYK"
     primer_bind     complement(55..76)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(316..335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     663..684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cttgccncca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
       61 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      121 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      181 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      241 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      301 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      361 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      421 ctggggcaca agctggagta caaccacaac agccacaacg tctatatcat ggccgacaag
      481 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      541 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      601 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      661 cacatggtcc tgctggagtt cgtgaccgcc gccgggatcg ctctcggcat ggacgagctg
      721 tacaagtccg gactcanatc ttgcactgaa cccctaggcc tg
//
