LOCUS       sequence-287370-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP12 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP12
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagttg gcatgtccgc cgctagactg actccttgtg
      121 gtactggcac ctctactgac gtggtgtaca gggccttcga catctacaac gacaaggtgg
      181 ccggattcgc taagttcctg aagaccaact gctgccgctt ccaggagaag gacgaagacg
      241 acaacctgat cgactcttac ttcgtggtga agaggcacac cttcagcaac taccagcacg
      301 aggaaaccat ctacaacctg ctgaaggact gccccgccgt ggctaagcac gacttcttca
      361 agttccgcat cgacggtgac atggtgcctc acatcagccg ccagaggctg accaagtaca
      421 ccatggccga cctggtgtac gctctgaggc acttcgacga gggcaactgc gacaccctga
      481 aggaaatcct ggtgacctac aactgctgcg acgacgacta cttcaacaag aaggactggt
      541 acgacttcgt ggagaaccct gacatcctga gagtgtacgc caacctgggc gaaagagtgc
      601 gtcaggctct gctgaagacc gtgcagttct gcgacgccat gcgtaacgct ggtatcgtgg
      661 gcgtgctgac cctggacaac caggacctga acggcaactg gtacgacttc ggagacttca
      721 tccagactac tccaggatct ggtgtgcctg tggtggactc ctactactct ctgctgatgc
      781 caatcctgac cctgaccagg gccctgaccg ctgagtctca cgtggacacc gacctgacca
      841 agccctacat caagtgggac ctgctgaagt acgacttcac cgaggaacgc ctgaagctgt
      901 tcgacagga
//
