LOCUS       sequence-287371-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP12 gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP12
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggg tattactgca
      121 gcacggtgtg aggggtgtac atggcctcgt agaattctgg ctcccagtac ctggaggtgt
      181 tgtcgttggt cagcatcacg ctgtacatgt ccagcatgtg tccggtcagt tcgtcgtgca
      241 gcttgcggat gtactgcagg tacaggtgga acacgtcagc gtactcctgg tttgggtgct
      301 tggtcagagg gtaagcgtcg atggccaggc tcacgaacct ttcgatcatc agggtgccgt
      361 cggtcttcac gatgtcgtcc acgaagcatc cagctcccag gattctagaa gggtcagggt
      421 atggcaggta cacgtagtcg tcaccctgct tcaccagcat ggtgtgctgg gagcagaact
      481 cgtgaggtcc cttggtcagg tcggtttcgg tccagcactt ggcctcgctc atgaacacgt
      541 tgttctggta gtacagcacg gacttgaagt tcttgatgct agccaccagg ccctggctgg
      601 cgtaggtaga gttgaagcac accacagcgt cgtcagacag gatcatcatg gagaagtgct
      661 tgcgcaggta ggcgtagaat tcgttcacga agtcggtgtc cacgtcacgg tttctgtaca
      721 ggcactcgta cagacggtgc tgcaggtttc tcacgtactt gtcagcgatc ttgttaccgt
      781 cggtagacag cagggcgttc acgttagcgg tcacggcctg gcagatgttg aacacggagt
      841 tagcgtaggc ggtggtagcg tctcca
//
