LOCUS       sequence-287374-        7978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BRD3
FEATURES             Location/Qualifiers
     source          1..7978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(709..711,712..714,715..1425)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(754..775)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1015..1034)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1362..1383
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1441..1465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3734..3758)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3766..3807
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3847..3866)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3891..3939
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(4142..4158)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4142..4158)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4155..4177)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4166..4182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4190..4196,4197..4214,4215..4220))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4235..4256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4373..4390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4544..5132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4624..4643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5303..6094,6095..6163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5926..5945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6164..6268)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(6680..7858)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6689..7627
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6712..6732)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gactctagag gatccctacc ggtgatatcc tcgagaccat ggtgagcaag
      721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      901 ttcacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1141 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg
     1201 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1321 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggctc gagcccatca
     1441 acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa ccaattctct aaggaaatac
     1501 ttaaccatgg tcgactggat ccggtaccga attctcatgt ccaccgccac gacagtcgcc
     1561 cccgcgggga tcccggcgac cccgggccct gtgaacccac cccccccgga ggtctccaac
     1621 cccagcaagc ccggccgcaa gaccaaccag ctgcagtaca tgcagaatgt ggtggtgaag
     1681 acgctctgga aacaccagtt cgcctggccc ttctaccagc ccgtggacgc aatcaaattg
     1741 aacctgccgg attatcataa aataattaaa aacccaatgg atatggggac tattaagaag
     1801 agactagaaa ataattatta ttggagtgca agcgaatgta tgcaggactt caacaccatg
     1861 tttacaaatt gttacattta taacaagccc acagatgaca tagtgctaat ggcccaagct
     1921 ttagagaaaa tttttctaca aaaagtggcc cagatgcccc aagaggaagt tgaattatta
     1981 ccccctgctc caaagggcaa aggtcggaag ccggctgcgg gagcccagag cgcaggtaca
     2041 cagcaagtgg cggccgtgtc ctctgtctcc ccagcgaccc cctttcagag cgtgcccccc
     2101 accgtctccc agacgcccgt catcgctgcc acccctgtac caaccatcac tgcaaacgtc
     2161 acgtcggtcc cagtcccccc agctgccgcc ccacctcctc ctgccacacc catcgtcccc
     2221 gtggtccctc ctacgccgcc tgtcgtcaag aaaaagggcg tgaagcggaa agcagacaca
     2281 accactccca cgacgtcggc catcactgcc agccggagtg agtcgccccc gccgttgtca
     2341 gaccccaagc aggccaaagt ggtggcccgg cgggagagtg gtggccgccc catcaagcct
     2401 cccaagaagg acctggagga cggcgaggtg ccccagcacg caggcaagaa gggcaagctg
     2461 tcggagcacc tgcgctactg cgacagcatc ctcagggaga tgctatccaa gaagcacgcg
     2521 gcctacgcct ggcccttcta caagccagtg gatgccgagg ccctggagct gcacgactac
     2581 cacgacatca tcaagcaccc gatggacctc agcaccgtga aaaggaagat ggatggccga
     2641 gagtacccag acgcacaggg ctttgctgct gatgtccggc tgatgttctc gaattgctac
     2701 aaatacaatc ccccagacca cgaggttgtg gccatggccc ggaagctcca ggacgtgttt
     2761 gagatgaggt ttgccaagat gccagatgag cccgtggagg caccggcgct gcctgccccc
     2821 gcggccccca tggtgagcaa gggcgctgag agcagccgta gcagtgagga gagctcttcg
     2881 gactcaggca gctcggactc ggaggaggag cgggccacca ggctggcgga gctgcaggag
     2941 cagctgaagg ccgtgcacga gcagctggcc gccctgtctc aggccccagt aaacaaacca
     3001 aagaagaaga aggagaagaa ggagaaggag aagaagaaga aggacaagga gaaggagaag
     3061 gagaagcaca aagtgaaggc cgaggaagag aagaaggcca aggtggctcc gcctgccaag
     3121 caggctcagc agaagaaggc tcctgccaag aaggccaaca gcacgaccac ggccggcaga
     3181 cagctgaaga aaggcggcaa gcaggcatct gcctcctacg actcagagga agaggaggag
     3241 ggcctgccca tgagctacga tgaaaagcgc cagcttagcc tggacatcaa ccggctgccc
     3301 ggggagaagc tgggccgggt agtgcacatc atccaatctc gggagccctc gctcagggac
     3361 tccaaccccg acgagataga aattgacttt gagactctga aacccaccac tttgcgggaa
     3421 ctggagagat atgtcaagtc ttgtttacag aaaaagcaaa ggaaaccgtt ctcagcaagc
     3481 gggaagaaac aggcagccaa gtcgaaagag gagctagctc aggaaaagaa gaaggagctg
     3541 gaaaagcgtc tgcaggatgt cagcgggcag ctgagcagca gcaagaagcc cgcccggaaa
     3601 gagaagcccg gctcagcacc ctcagggggc ccgtccaggc tcagcagcag cagctcctcc
     3661 gagtctggga gcagcagctc cagcgggtcc agctctgaca gcagtgactc agaattgctc
     3721 tcgagatatc tagacccagc tttcttgtac aaagtggttg ataacgggaa gcctatccct
     3781 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag
     3841 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     3901 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     3961 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4021 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4081 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggaagcttg gcgtaatcat
     4141 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4201 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcat ctgcagaatt
     6301 cggcttcccg gtcaacatcc agtacacctc cggcaccacc ggcttcccca agggcgccac
     6361 gctctcccac cacaacatcc tcaacaacgg ctactgggtg ggccgcacgg tcggctacac
     6421 ggagcaggac cgggtgtgtc tgccggtgcc cttctaccac tgcttcggca tggtcatggg
     6481 caacttgggc gccacctccc acgccgcctg catcgtcatc ccggccccgt ccttcgagcc
     6541 ggcggcgacg ctggaggcgg tgcagcggga gaggtgcacg tccctgtacg gcgtccccac
     6601 gaagccgaat tccaccacac tggactagat gcatgctcga gcggccgcca gtgtgatgga
     6661 tatctgcaga attcggcttt cacgacacct gaaatggaag aaaaaaactt tgaaccactg
     6721 tctgaggctt gagaatgaac caagatccaa actcaaaaag ggcaaattcc aaggagaatt
     6781 acatcaagtg ccaagctggc ctaacttcag tctccaccca ctcagtgtgg ggaaactcca
     6841 tcgcataaaa cccctccccc caacctaaag acgacgtact ccaaaagctc gagaactaat
     6901 cgaggtgcct ggacggcgcc cggtactccg tggagtcaca tgaagcgacg gctgaggacg
     6961 gaaaggccct tttcctttgt gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc
     7021 tccattttga gctccctgca gcaggaccgg gaagcggcca tctttccgct cacgcaactg
     7081 gtgccgaccg ggccagcctt gccgcccggg gcggggcgat acacggcggc gcgaggacag
     7141 gcaccagagc aggccggcca gcttgagact acccccgtcc gattctcggt ggtcgcgctc
     7201 gcaggccccg cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg
     7261 ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc
     7321 agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag
     7381 cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc
     7441 aggcctcaac tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc
     7501 acccacttcc aacccgaagc tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa
     7561 ttcaaggcac gcaagggcca taacccgtaa agaggccagg cccgcgggaa ccacacacgg
     7621 cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg
     7681 cacttatata cggttctccc ccaccctcgg gaaaaaggcg gagccagtac acggcatcac
     7741 tttcccagtt taccccgcgc caccttctct aggcaccggt tcaattgccg acccctcccc
     7801 ccaacttctc ggggactgtg ggcgatgtgc gctctgccca ctgacgggca ccggagccaa
     7861 gccgaattcc agcacactgg cggccgttac tagtggatcc gagctcggta cctgaagaat
     7921 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
