LOCUS       sequence-287377-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             310..333
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             370..393
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(572..589)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gtgtcgtgag gatctatttc cggtgaattc gccgccacca tgagcaagat gagcgacgta
       61 aaatgcacgt cagttgttct cttgagcgtc ctgcaacaac tcagagtgga gtctagttcc
      121 aagctctggg ctcaatgcgt tcagcttcac aatgacattc tgttggcgaa agataccacg
      181 gaggcattcg agaagatggt ctcacttctg agcgttctcc tgtcaatgca aggggcggtc
      241 gatatcaata aattgtgtga ggaaatgctc gacaaccggg cgacattgca gctcgaaggc
      301 ggcgggggat ggagccatcc acaattcgag aaaggcggtg gttcaggagg aggtagcggg
      361 ggtggatcat ggtcacatcc gcagtttgaa aagtaaggat cccgcccctc tccctccccc
      421 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
      481 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
      541 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
      601 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
      661 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
      721 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
      781 gttgtggaaa gagtcaa
//
