LOCUS       sequence-287380-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: 
            pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtgtcgtgag gatctatttc cggtgaattc gccgccacca tgtctggttt taggaaaatg
       61 gcgttcccca gcggtaaagt tgaaggatgt atggtccaag taacctgtgg taccactacc
      121 cttaatgggc tttggttgga cgacgtagtc tactgccccc gacacgtaat ctgcactagt
      181 gaggatatgc ttaatcccaa ttacgaagac cttttgattc ggaaatccaa tcacaacttc
      241 ctggtccaag cgggcaacgt ccaactcagg gttattggac atagtatgca gaattgcgta
      301 ctgaagctca aagtcgatac tgcaaacccc aagacgccca agtataaatt cgtccgaatc
      361 caaccaggcc aaacattttc cgtattggct tgctataatg gaagccccag cggtgtctac
      421 caatgtgcaa tgagaccaaa ctttacgata aagggttcat ttctgaacgg ctctgccggt
      481 tccgttggtt ttaacatcga ctatgactgt gtatcctttt gctacatgca ccatatggaa
      541 ctccctaccg gtgtccacgc cggtacagat ctggaaggaa atttctacgg tccgttcgtt
      601 gaccggcaaa ccgcgcaagc ggctggaacc gacacaacga ttacagtgaa tgtgctcgcg
      661 tggctgtacg cagcagtcat aaacggagac aggtggtttc tgaaccgatt tacgacgact
      721 ctcaatgact tcaaccttgt tgcgatgaag tacaattacg agccactcac ccaggaccat
      781 gttgatatcc tgggtcccct cagtgcccag acagggatcg cagttctcga tatgtgcgcg
      841 tcactgaagg agcttctcca a
//
