LOCUS       sequence-287406-        6666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Redox-Sensitive Red Fluorescent Protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Fan Y, Chen Z, Ai HW
  TITLE     Monitoring redox dynamics in living cells with a redox-sensitive red
            fluorescent protein.
  JOURNAL   Anal Chem. 2015 Mar 3;87(5):2802-10. doi: 10.1021/ac5041988. Epub 
            2015 Feb 20.
  PUBMED    25666702
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac5041988";
            journalName: "Anal Chem"; date: "2015-03-3- 3"; volume: "87"; issue:
            "5"; pages: "2802-10"
COMMENT     Sequence Label: pcDNA3-rxRFP
FEATURES             Location/Qualifiers
     source          1..6666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(377..395)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(378..395)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(415..432)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    421..645
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      691..1119
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(778..797)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     988..1009
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1128..1148)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1133..1462
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1313..1448
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1375..1394
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1529..2323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1583..1602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2193..2212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2497..2618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2534..2553)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2588..2607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2667..2683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2667..2683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2680..2702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2691..2707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2715..2721,2722..2739,2740..2745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2760..2781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2898..2915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3069..3657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3149..3168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3828..4619,4620..4688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4451..4470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4689..4793)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4868..4887)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5059..5438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5439..5642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5593..5613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5687..5706
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5687..5705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5722..5731
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5740..5757
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5761..5793
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(5797..5805,5806..5820)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     6564..6583
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgttagggca ttgttcgact ttaacggaaa tgacgaggaa gatttaccat ttaagaaagg
       61 ggatatccta cgtataagag ataaacccga ggaacaatgg tggaatgcgg aagacagtga
      121 aggtaagcga ggcatgattc cggtgcctta cgtagagaaa tattaactcg agggcggcag
      181 cggcggcgct gagtatgtta gggcattgtt cgactttaac ggaaatgacg aggaagattt
      241 accatttaag aaaggggata tcctacgtat aagagataaa cccgaggaac aatggtggaa
      301 tgcggaagac agtgaaggta agcgaggcat gattccggtg ccttacgtag agaaatatta
      361 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
      421 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      481 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      541 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      601 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
      661 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
      721 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      781 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      841 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      901 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      961 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     1021 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     1081 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     1141 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1201 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1261 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     1321 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     1381 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     1441 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     1501 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     1561 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     1621 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     1681 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     1741 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     1801 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     1861 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     1921 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     1981 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     2041 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     2101 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     2161 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     2221 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     2281 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     2341 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     2401 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     2461 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     2521 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2581 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     2641 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2701 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2761 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2821 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2881 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2941 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3001 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3061 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3961 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4141 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4201 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4261 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4321 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4441 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4681 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4741 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4801 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     4861 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     4921 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     4981 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     5041 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     5101 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     5161 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     5221 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     5281 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     5341 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     5401 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     5461 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     5521 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     5581 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     5641 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
     5701 atagggagac ccaagcttgc cgccaccatg ggaggttctc atcatcatca tcatcatggt
     5761 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
     5821 gatccgagct cgagcgagct tagttgcgct ataggtcggc tgagctcacc cgtggtttcc
     5881 gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaaggg gctgaggctg
     5941 aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
     6001 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca caacgaggac
     6061 tacaccatcg tggaacagtg cgaacgcgcc gagggccgcc actccaccgg cgacatggac
     6121 gagctgtaca agggaggtac aggcgggagt ctggtgagca agggcgagga ggataacatg
     6181 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     6241 gagttcgaga tcgagggcga gggcgagggc cgcccctacg aggcctttca gaccgctaag
     6301 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     6361 atgtacggct ccaaggccta cattaagcac ccagccgaca tccccgacta cttcaagctg
     6421 tccttccccg agggcttcag gtgggagcgc gtgatgaact tcgaggacgg cggcattatt
     6481 cacgttaacc aggactcctc cctgcaggac ggcgtattca tctacaaggt gaagctgcgc
     6541 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggct
     6601 acgcgtgacc aaaggtgctg cagtgtggct taagctggaa ttcggcggca gcggcggcgc
     6661 tgagta
//
