LOCUS       sequence-287414-        9999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Krupka N, Strappe P, Gotz J, Ittner LM
  TITLE     Gateway-compatible lentiviral transfer vectors for ubiquitin 
            promoter driven expression of fluorescent fusion proteins.
  JOURNAL   Plasmid. 2010 May;63(3):155-60. doi: 10.1016/j.plasmid.2010.01.002. 
            Epub 2010 Feb 2.
  PUBMED    20132838
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.01.002"; journalName: "Plasmid"; date: 
            "2010-05"; volume: "63"; issue: "3"; pages: "155-60"
COMMENT     Sequence Label: pLVU/GFP
FEATURES             Location/Qualifiers
     source          1..9999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2858..4069
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3906..3925
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4129..4253
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4278..4283,4284..4301,4302..4308)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4362..5021
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4428..4447)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5363..5668
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5547..5566
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5709..5833)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(5886..5888,5889..5891,5892..6605)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5931..5952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6192..6211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6539..6560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6642..6662)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6647..6976
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6827..6962
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7024..7071
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7090..7488
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7575..7808
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7880..8001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7917..7936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7971..7990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8041..8176
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8196..8215)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8197..8215)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8225..8242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8225..8241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8234..8256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8383..8838
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8470..8489)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8680..8701
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8864..8968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8969..9037,9038..9829)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9187..9206)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggatctggc ctccgcgccg ggttttggcg
     2881 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     2941 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     3001 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     3061 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     3121 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     3181 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     3241 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     3301 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     3361 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     3421 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     3481 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     3541 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     3601 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     3661 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     3721 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     3781 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     3841 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     3901 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     3961 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     4021 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttggta
     4081 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatcaac aagtttgtac
     4141 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     4201 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     4261 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     4321 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     4381 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     4441 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     4501 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     4561 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     4621 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     4681 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     4741 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     4801 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     4861 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     4921 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     4981 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
     5041 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     5101 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     5161 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     5221 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     5281 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     5341 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     5401 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     5461 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     5521 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     5581 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     5641 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     5701 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     5761 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     5821 tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt
     5881 tcgaaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     5941 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     6001 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     6061 ccaccctcgt gaccaccttc acctacggcg tgcagtgctt cgcccgctac cccgaccaca
     6121 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     6181 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     6241 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     6301 ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga
     6361 agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc
     6421 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     6481 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     6541 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     6601 agtaacgtac cggttagtaa tgagtttgga attaattctg tggaatgtgt gtcagttagg
     6661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6721 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6781 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6841 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6901 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6961 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7021 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     7081 aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     7141 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     7201 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     7261 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     7321 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     7381 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     7441 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc acaattcgag
     7501 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7621 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7681 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7741 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7801 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7861 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7981 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8041 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8101 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8161 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     8221 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     8281 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     8341 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     8401 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     8461 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     8521 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     8581 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8641 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8701 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8761 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8821 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8881 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8941 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9001 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9061 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9121 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9181 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9241 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9301 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9361 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9421 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9481 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9541 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9601 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     9661 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9721 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9781 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9841 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9901 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9961 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
