LOCUS       sequence-287473-       11747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS
FEATURES             Location/Qualifiers
     source          1..11747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1929..3422
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3519..3539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3604..4192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3657..3677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4075..4086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4195..4211)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4196..4212)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4302..4535
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4560..4578)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4561..4578)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4868..4887)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5101..5120)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5220..5242
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5280..5298)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5366..5470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5471..5539,5540..6331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5689..5708)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6502..7090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6991..7010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7244..7261
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7331..7351)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7336..7665
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7516..7651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8799..8864
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8994..9014
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9439..9573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9476..9495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9530..9549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9741..10374
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10421..10546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11043..11276
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11461..11505
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatgtcc cgagccggta gctgggacat ggacggcctg cgagcggacg
      841 gaggcggagc tggaggagct cccgcatcaa gtagtagctc aagtgtggcc gctgccgcgg
      901 ctagcggaca atgtaggggg tttttgtcag cgcctgtctt tgcgggcaca cattccggga
      961 gggccgctgc tgcggctgct gctgctgctg cagccgcggc tgcagctgca gctgcggccg
     1021 cagcagctgc cgctagtgga tttgcctatc ctgggacgag tgagcgcact ggttcatcat
     1081 cctcatcttc atccagtgcg gtagtcgccg ctcggccaga agcaccacct gcaaaagagt
     1141 gtccggcccc gacgccagct gcagcagcag cggcaccgcc ttcagctcct gcgggtaccg
     1201 gaggcgggat ggtttccaaa ggagaggagg ataatatggc tataattaaa gagtttatgc
     1261 ggttcaaggt gcatatggag ggatctgtga acggtcacga gttcgaaatc gagggcgaag
     1321 gcgaggggcg accctatgaa gggacacaga cggccaaact gaaggtgacc aagggtggcc
     1381 ccctgccttt cgcctgggac atcctgtcac cccagtttat gtacggctct aaggcttacg
     1441 ttaagcaccc tgccgatatt ccggactact tgaaactgag cttccctgaa gggttcaagt
     1501 gggaacgggt aatgaatttt gaggatggcg gtgtagtcac agttacccag gacagctctc
     1561 ttcaggacgg agaatttatc tataaggtta aacttcgggg cactaacttc ccatcagacg
     1621 gccccgtgat gcagaagaaa actatgggct gggaagccag ctcagaacgc atgtatcccg
     1681 aggacggagc actgaaggga gaaatcaagc agcgactcaa gctgaaggat gggggacatt
     1741 atgatgctga ggtcaaaacc acctataagg ccaagaaacc cgttcagctt cctggggcat
     1801 acaacgtgaa tatcaaactg gatattacat cccataacga agactatacc atcgtggaac
     1861 agtacgagcg ggccgagggc agacatagca cagggggcat ggatgaattg tacaaggcgg
     1921 ccacgcgtat gaagatggac aaaaagacta tagtttggtt tagaagagac ctaaggattg
     1981 aggataatcc tgcattagca gcagctgctc acgaaggatc tgtttttcct gtcttcattt
     2041 ggtgtcctga agaagaagga cagttttatc ctggaagagc ttcaagatgg tggatgaaac
     2101 aatcacttgc tcacttatct caatccttga aggctcttgg atctgacctc actttaatca
     2161 aaacccacaa cacgatttca gcgatcttgg attgtatccg cgttaccggt gctacaaaag
     2221 tcgtctttaa ccacctctat gatcctgttt cgttagttcg ggaccatacc gtaaaggaga
     2281 agctggtgga acgtgggatc tctgtgcaaa gctacaatgg agatctattg tatgaaccgt
     2341 gggagatata ctgcgaaaag ggcaaacctt ttacgagttt caattcttac tggaagaaat
     2401 gcttagatat gtcgattgaa tccgttatgc ttcctcctcc ttggcggttg atgccaataa
     2461 ctgcagcggc tgaagcgatt tgggcgtgtt cgattgaaga actagggctg gagaatgagg
     2521 ccgagaaacc gagcaatgcg ttgttaacta gagcttggtc tccaggatgg agcaatgctg
     2581 ataagttact aaatgagttc atcgagaagc agttgataga ttatgcaaag aacagcaaga
     2641 aagttgttgg gaattctact tcactacttt ctccgtatct ccatttcggg gaaataagcg
     2701 tcagacacgt tttccagtgt gcccggatga aacaaattat atgggcaaga gataagaaca
     2761 gtgaaggaga agaaagtgca gatctttttc ttaggggaat cggtttaaga gagtattctc
     2821 ggtatatatg tttcaacttc ccgtttactc acgagcaatc gttgttgagt catcttcggt
     2881 ttttcccttg ggatgctgat gttgataagt tcaaggcctg gagacaaggc aggaccggtt
     2941 atccgttggt ggatgccgga atgagagagc tttgggctac cggatggatg cataacagaa
     3001 taagagtgat tgtttcaagc tttgctgtga agtttcttct ccttccatgg aaatggggaa
     3061 tgaagtattt ctgggataca cttttggatg ctgatttgga atgtgacatc cttggctggc
     3121 agtatatctc tgggagtatc cccgatggcc acgagcttga tcgcttggac aatcccgcgt
     3181 tacaaggcgc caaatatgac ccagaaggtg agtacataag gcaatggctt cccgagcttg
     3241 cgagattgcc aactgaatgg atccatcatc catgggacgc tcctttaacc gtactcaaag
     3301 cttctggtgt ggaactcgga acaaactatg cgaaacccat tgtagacatc gacacagctc
     3361 gtgagctact agctaaagct atttcaagaa cccgtgaagc acagatcatg atcggagcag
     3421 cagcccggga tccaccggtc gccaccggtt cagggagcgg atccggctca gggagtgcgg
     3481 ccggaactcc cacctgcaac atgcgtgacg gaggcggtcc aaaaaagaag agaaaggtat
     3541 gactgaggcc gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc
     3601 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3661 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3721 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3781 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3841 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3901 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3961 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4021 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4081 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4141 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     4201 cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc
     4261 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     4321 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     4381 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     4441 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     4501 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     4561 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     4621 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     4681 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     4741 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     4801 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     4861 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     4921 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     4981 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     5041 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     5101 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5161 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5221 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     5281 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggaac
     6121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6181 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7141 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7201 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7261 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7321 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7381 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7441 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7501 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7561 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7621 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
     7681 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     7741 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     7801 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     7861 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     7921 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     7981 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     8041 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     8101 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     8161 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     8221 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     8281 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     8341 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     8401 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     8461 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     8521 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     8581 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
     8641 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
     8701 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
     8761 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
     8821 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
     8881 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
     8941 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
     9001 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
     9061 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
     9121 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
     9181 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
     9241 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
     9301 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
     9361 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     9421 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9481 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9541 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
     9601 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
     9661 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
     9721 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
     9781 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     9841 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     9901 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     9961 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
    10021 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
    10081 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
    10141 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
    10201 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
    10261 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
    10321 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
    10381 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
    10441 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
    10501 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
    10561 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
    10621 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
    10681 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    10741 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    10801 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    10861 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
    10921 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10981 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    11041 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    11101 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    11161 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    11221 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    11281 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    11341 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    11401 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    11461 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    11521 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    11581 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    11641 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    11701 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaa
//
