LOCUS       sequence-287490-       10259 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10259)
  AUTHORS   Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S,
            Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, 
            Ibrahim DM, Hnisz D
  TITLE     Unblending of Transcriptional Condensates in Human Repeat Expansion 
            Disease.
  JOURNAL   Cell. 2020 May 28;181(5):1062-1079.e30. doi: 
            10.1016/j.cell.2020.04.018. Epub 2020 May 7.
  PUBMED    32386547
REFERENCE   2  (bases 1 to 10259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.04.018"; journalName: "Cell"; date: 
            "2020-05-28- 28"; volume: "181"; issue: "5"; pages: "1062-1079.e30"
COMMENT     Sequence Label: pHR-HOXD13(+7A)-IDR-YFP-NLS
FEATURES             Location/Qualifiers
     source          1..10259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(1269..1271,1272..1274,1275..1985)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1314..1335)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1575..1594)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1922..1943
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1995..2015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2116..2704
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2169..2189)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2587..2598)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2707..2723)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2708..2724)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2814..3047
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3072..3090)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3073..3090)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3380..3399)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3613..3632)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3732..3754
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3792..3810)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3878..3982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3983..4051,4052..4843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4201..4220)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5014..5602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5503..5522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5756..5773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5843..5863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5848..6177
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6028..6163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7311..7376
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7506..7526
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7951..8085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7988..8007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8042..8061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8253..8886
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8933..9058
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9555..9788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9973..10017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatgtcc cgagccggta gctgggacat ggacggcctg cgagcggacg
      841 gaggcggagc tggaggagct cccgcatcaa gtagtagctc aagtgtggcc gctgccgcgg
      901 ctagcggaca atgtaggggg tttttgtcag cgcctgtctt tgcgggcaca cattccggga
      961 gggccgctgc tgcggctgct gctgctgctg cagccgcggc ggcagctgca gctgcggccg
     1021 ctgccgctag tggatttgcc tatcctggga cgagtgagcg cactggttca tcatcctcat
     1081 cttcatccag tgcggtagtc gccgctcggc cagaagcacc acctgcaaaa gagtgtccgg
     1141 ccccgacgcc agctgcagca gcagcggcac cgccttcagc tcctgcgggt accggaggcg
     1201 ggatggtttc caaaggagag gaggataata tggctataat taaagagttt atgcggttca
     1261 aggtgcatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1321 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1381 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1441 ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc
     1501 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1561 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1621 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1681 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1741 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1801 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1861 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     1921 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1981 acaagggagg cggtccaaaa aagaagagaa aggtatgagc ggccgcaact cccacctgca
     2041 acatgcgtga ctgactgagg ccgcgactct agagtcgacc tgcaggcatg caagcttgat
     2101 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2161 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2221 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2281 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2341 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2401 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2461 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2521 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2581 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2641 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2701 ccgcatcgat accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat
     2761 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     2821 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     2881 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2941 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     3001 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
     3061 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     3121 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
     3181 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
     3241 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
     3301 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     3361 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     3421 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     3481 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     3541 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     3601 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3661 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3721 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3781 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3841 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3901 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3961 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4021 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4081 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4141 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4201 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4261 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4321 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4381 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4441 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4501 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     4561 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4621 agttgcagga acacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4681 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4741 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4801 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4861 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4921 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5161 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5341 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5401 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5461 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5521 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5581 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5641 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5701 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5761 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5821 gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5941 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6001 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6181 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     6241 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     6301 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     6361 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     6421 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     6481 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     6541 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     6601 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     6661 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     6721 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
     6781 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     6841 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
     6901 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
     6961 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
     7021 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     7081 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
     7141 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
     7201 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
     7261 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     7321 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     7381 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     7441 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     7501 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     7561 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     7621 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
     7681 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
     7741 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
     7801 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     7861 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     7921 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     7981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8041 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     8101 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     8161 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     8221 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     8281 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     8341 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     8401 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     8461 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     8521 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     8581 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     8641 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     8701 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8881 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8941 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     9001 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     9061 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     9121 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     9181 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     9241 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     9301 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     9361 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     9421 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9481 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     9541 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     9601 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     9661 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     9721 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     9781 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     9841 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     9901 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     9961 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    10021 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    10081 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    10141 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    10201 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaa
//
