LOCUS       sequence-28751-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Lin CW, Jao CY, Ting AY
  TITLE     Genetically encoded fluorescent reporters of histone methylation in 
            living cells.
  JOURNAL   J Am Chem Soc. 2004 May 19. 126(19):5982-3.
  PUBMED    15137760
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2004 May 19. 126(19):5982-3."
COMMENT     Sequence Label: pcDNA3-K27 histone methylation reporter
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..337)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(128..149)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     477..496
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acctacatag gtgaactata gaatagggcc ctctagatgc atgctcgagc ggccgccagt
       61 gtgatggata tctgcagaat tcttacttgt acagctcgtc catgccgaga gtgatcccgg
      121 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      181 gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      241 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      301 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      361 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      421 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      481 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      541 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cacatcaggc
      601 cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      661 tgaacttcag ggtcagcttg ccgtaagtgg catcgccctc gccctcgccg gacacgct
//
