LOCUS       sequence-287757-        1962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 E (envelope).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1962)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046935
FEATURES             Location/Qualifiers
     source          1..1962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             333..353
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             354..383
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      500..1088
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     989..1008
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1194..1853
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1260..1279)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1924..1958
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg tactcattcg
      121 tttcggaaga gacaggtacg ttaatagtta atagcgtact tctttttctt gctttcgtgg
      181 tattcttgct agttacacta gccatcctta ctgcgcttcg attgtgtgcg tactgctgca
      241 atattgttaa cgtgagtctt gtaaaacctt ctttttacgt ttactctcgt gttaaaaatc
      301 tgaattcttc tagagttcct gatcttctgg tcgagaacct gtactttcaa agcgagcaga
      361 agcttatttc cgaagaagat ctctaaccgg cttatcggtc agtttcacct gatttacgta
      421 aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg actgtccacg
      481 acgctatacc caaaagaaat tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      541 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      601 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      661 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      721 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      781 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      841 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      901 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      961 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1021 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1081 tatggaaatt gatcgggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     1141 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
     1201 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     1261 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     1321 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     1381 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg
     1441 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     1501 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     1561 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     1621 ttttcgtttc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     1681 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1741 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1801 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatttgata
     1861 tcgagctcgc ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat
     1921 ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gt
//
