LOCUS       sequence-287758-        1779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1779)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046725
FEATURES             Location/Qualifiers
     source          1..1779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             150..170
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             171..200
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      317..905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     806..825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1011..1670
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1077..1096)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1741..1775
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg agcgctgatg
      121 cgcaaagttt tctcaatggt ttcgccgtag agaacctgta ctttcaaagc gagcagaagc
      181 ttatttccga agaagatctc taaccggctt atcggtcagt ttcacctgat ttacgtaaaa
      241 acccgcttcg gcgggttttt gcttttggag gggcagaaag atgaatgact gtccacgacg
      301 ctatacccaa aagaaattga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      481 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      901 ggaaattgat cgggcacgta agaggttcca actttcacca taatgaaata agatcactac
      961 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     1021 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     1081 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     1141 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     1201 cccgcctgat gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga
     1261 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     1321 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     1381 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     1441 tcgtttcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     1501 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     1561 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     1621 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttgatatcg
     1681 agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     1741 ttcagaacgc tcggttgccg ccgggcgttt tttattggt
//
