LOCUS       sequence-287759-        1767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1767)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046761
FEATURES             Location/Qualifiers
     source          1..1767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..164
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      305..893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     794..813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             999..1658
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1065..1084)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1729..1763
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg gctgatgcac
      121 aatcgttttt aaacgggttt gcggagaacc tgtactttca aagcgactac aaggatgatg
      181 atgacaaata accggcttat cggtcagttt cacctgattt acgtaaaaac ccgcttcggc
      241 gggtttttgc ttttggaggg gcagaaagat gaatgactgt ccacgacgct atacccaaaa
      301 gaaattgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      361 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      421 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      481 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      541 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      601 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      661 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      721 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      781 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      841 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaattgatcg
      901 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      961 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1021 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1081 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1141 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1201 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1261 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1321 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1381 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtttcagcca
     1441 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1501 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1561 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1621 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     1681 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     1741 ggttgccgcc gggcgttttt tattggt
//
