LOCUS       sequence-287765-        2766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 endoRNAse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2766)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046740
FEATURES             Location/Qualifiers
     source          1..2766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1149..1169
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1170..1187
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      1304..1892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1793..1812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1998..2657
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2064..2083)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2728..2762
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg tccttagaaa
      121 atgtcgcctt caacgttgta aacaaaggcc actttgatgg tcagcaaggg gaggttcctg
      181 tgtcaatcat taacaacact gtatacacaa aggtcgacgg cgttgacgtg gaactcttcg
      241 aaaataaaac cactcttccg gttaacgttg catttgagct atgggcgaaa cgtaacatca
      301 aaccggtgcc agaagtaaaa attttgaaca acctgggtgt ggatatcgct gctaatacgg
      361 ttatttggga ctataagaga gatgcgccgg cacatatctc gaccattggt gtttgcagta
      421 tgactgacat cgctaaaaaa cccaccgaaa ccatctgtgc cccgctgacg gtattcttcg
      481 atggccgcgt cgatggtcag gtagacctgt tccggaacgc acgcaatggc gtactgataa
      541 ctgagggatc tgtgaagggc ctgcaaccga gcgttggtcc gaaacaggct agcctgaacg
      601 gtgttaccct gatcggcgaa gcggtcaaaa ctcagtttaa ctactataag aaagtggacg
      661 gtgttgtgca gcagctgccc gaaacgtact tcacacagtc tcgtaacctg caagaattta
      721 aacctcgttc tcagatggaa atcgatttcc tggagctggc tatggacgag tttatcgaac
      781 gctacaaact agaaggctac gccttcgaac acattgtcta tggcgacttc tcccacagcc
      841 agctgggtgg actgcatctg ttgataggtc ttgcgaaacg tttcaaagaa tccccgtttg
      901 agttagaaga ttttattccg atggactcta ctgttaaaaa ttacttcatc accgatgctc
      961 aaaccggcag cagcaaatgc gtttgttctg tcatcgatct gctgctcgat gacttcgtgg
     1021 aaatcattaa gtcccaggat ctgtctgttg tttcaaaagt ggtaaaagta actatcgact
     1081 ataccgagat ttcctttatg ctgtggtgca aggatggcca cgttgaaact ttctacccaa
     1141 aattgcagga gaacctgtac tttcaaagcc accatcatca ccatcattaa ccggcttatc
     1201 ggtcagtttc acctgattta cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg
     1261 cagaaagatg aatgactgtc cacgacgcta tacccaaaag aaattgagat cctttttttc
     1321 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1381 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1441 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1501 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1561 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1621 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1681 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1741 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1801 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1861 gatgctcgtc aggggggcgg agcctatgga aattgatcgg gcacgtaaga ggttccaact
     1921 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1981 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     2041 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     2101 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     2161 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
     2221 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     2281 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     2341 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     2401 ctaaagggtt tattgagaat atgtttttcg tttcagccaa tccctgggtg agtttcacca
     2461 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     2521 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     2581 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     2641 ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta
     2701 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
     2761 attggt
//
