LOCUS       sequence-287785-        1908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1908)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046899
FEATURES             Location/Qualifiers
     source          1..1908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             291..311
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             312..329
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      446..1034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     935..954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1140..1799
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1206..1225)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1870..1904
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg tttcatctcg
      121 ttgactttca ggttactata gcagagatat tactaattat tatgaggact tttaaagttt
      181 ccatttggaa tcttgattac atcataaacc tcataattaa aaatttatct aagtcactaa
      241 ctgagaataa atattctcaa ttagatgaag agcaaccaat ggagattgat gagaacctgt
      301 actttcaaag ccaccatcat caccatcatt aaccggctta tcggtcagtt tcacctgatt
      361 tacgtaaaaa cccgcttcgg cgggtttttg cttttggagg ggcagaaaga tgaatgactg
      421 tccacgacgc tatacccaaa agaaattgag atcctttttt tctgcgcgta atctgctgct
      481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
      601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
      841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
      961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1021 ggagcctatg gaaattgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     1081 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     1141 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1201 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1261 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1321 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
     1381 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1441 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1501 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1561 atatgttttt cgtttcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1621 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1681 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1741 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     1801 ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc agaactccat
     1861 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggt
//
