LOCUS       sequence-287794-        2403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 M (membrane).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2403)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046739
FEATURES             Location/Qualifiers
     source          1..2403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             774..794
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             795..824
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      941..1529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1430..1449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1635..2294
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1701..1720)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2365..2399
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg gcagattcca
      121 acggtactat taccgttgaa gagcttaaaa agctccttga acaatggaac ctagtaatag
      181 gtttcctatt ccttacatgg atttgtcttc tacaatttgc ctatgccaac aggaataggt
      241 ttttgtatat aattaagtta attttcctct ggctgttatg gccagtaact ttagcttgtt
      301 ttgtgcttgc tgctgtttac agaataaatt ggatcaccgg tggaattgct atcgcaatgg
      361 cttgtcttgt aggcttgatg tggctcagct acttcattgc ttctttcaga ctgtttgcgc
      421 gtacgcgttc catgtggtca ttcaatccag aaactaacat tcttctcaac gtgccactcc
      481 atggcactat tctgaccaga ccgcttctag aaagtgaact cgtaatcgga gctgtgatcc
      541 ttcgtggaca tcttcgtatt gctggacacc atctaggacg ctgtgacatc aaggacctgc
      601 ctaaagaaat cactgttgct acatcacgaa cgctttctta ttacaaattg ggagcttcgc
      661 agcgtgtagc aggtgactca ggttttgctg catacagtcg ctacaggatt ggcaactata
      721 aattaaacac agaccattcc agtagcagtg acaatattgc tttgcttgta caggagaacc
      781 tgtactttca aagcgagcag aagcttattt ccgaagaaga tctctaaccg gcttatcggt
      841 cagtttcacc tgatttacgt aaaaacccgc ttcggcgggt ttttgctttt ggaggggcag
      901 aaagatgaat gactgtccac gacgctatac ccaaaagaaa ttgagatcct ttttttctgc
      961 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1021 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1081 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1141 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1201 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1261 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1321 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1381 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1441 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1501 gctcgtcagg ggggcggagc ctatggaaat tgatcgggca cgtaagaggt tccaactttc
     1561 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     1621 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     1681 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     1741 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     1801 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
     1861 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     1921 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     1981 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     2041 aagggtttat tgagaatatg tttttcgttt cagccaatcc ctgggtgagt ttcaccagtt
     2101 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     2161 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     2221 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     2281 agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga tccagtaatg
     2341 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     2401 ggt
//
