LOCUS       sequence-287800-        1914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1914)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046980
FEATURES             Location/Qualifiers
     source          1..1914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             291..311
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             312..335
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      452..1040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     941..960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1146..1805
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1212..1231)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1876..1910
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg ttccatctgg
      121 tcgactttca agttactatt gctgaaatat tactgatcat catgcgtacc ttcaaggtat
      181 cgatctggaa ccttgattac attatcaatc tcatcattaa aaacctgagt aaatccctga
      241 cggagaacaa atattctcag ttggatgaag aacagcctat ggagatcgac gagaacctgt
      301 actttcaaag cgactacaag gatgatgatg acaaataacc ggcttatcgg tcagtttcac
      361 ctgatttacg taaaaacccg cttcggcggg tttttgcttt tggaggggca gaaagatgaa
      421 tgactgtcca cgacgctata cccaaaagaa attgagatcc tttttttctg cgcgtaatct
      481 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      541 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      601 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      661 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      721 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      781 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      841 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      901 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      961 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1021 gggggcggag cctatggaaa ttgatcgggc acgtaagagg ttccaacttt caccataatg
     1081 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     1141 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     1201 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     1261 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     1321 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
     1381 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     1441 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     1501 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     1561 ttgagaatat gtttttcgtt tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     1621 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     1681 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     1741 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     1801 cgtaatttga tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa
     1861 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggt
//
