LOCUS       sequence-287805-        1776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1776)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046960
FEATURES             Location/Qualifiers
     source          1..1776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             150..170
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             171..197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      314..902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     803..822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1008..1667
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1074..1093)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1738..1772
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg agcgctgatg
      121 cgcaaagttt tctcaatggt ttcgccgtag agaacctgta ctttcaaagc tacccgtatg
      181 acgttcctga ttatgcctaa ccggcttatc ggtcagtttc acctgattta cgtaaaaacc
      241 cgcttcggcg ggtttttgct tttggagggg cagaaagatg aatgactgtc cacgacgcta
      301 tacccaaaag aaattgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      361 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      421 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      481 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      541 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      601 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      661 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      721 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      781 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      841 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      901 aattgatcgg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
      961 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1021 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1081 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1141 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1201 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
     1261 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1321 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1381 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1441 tttcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1501 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1561 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1621 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc
     1681 tcgcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     1741 agaacgctcg gttgccgccg ggcgtttttt attggt
//
