LOCUS       sequence-287811-        2634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose 
            methyltransferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2634)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046823
FEATURES             Location/Qualifiers
     source          1..2634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1005..1025
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1026..1055
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     rep_origin      1172..1760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1661..1680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1866..2525
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1932..1951)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2596..2630
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg tcaagccaag
      121 catggcagcc cggggtggcc atgccaaact tgtacaaaat gcagcgtatg ttactggaga
      181 agtgcgacct gcaaaattat ggtgattctg cgacgcttcc taaaggaatt atgatgaacg
      241 tagctaaata cactcagctg tgtcagtatc tcaacaccct gacactggct gtcccgtaca
      301 acatgcgcgt tatccacttc ggcgcaggct ccgataaagg tgttgcgccg ggcactgctg
      361 tactgcgtca gtggctgccg accggtactc tgctggttga ctctgacctc aatgattttg
      421 tgagtgacgc cgattccacc ctgatcggtg attgcgctac cgttcatact gcgaacaaat
      481 gggacctgat tatcagcgat atgtacgacc cgaagaccaa aaacgtaacc aaagaaaacg
      541 actcgaagga aggcttcttt acttatatct gtggtttcat tcagcagaaa ctggctcttg
      601 gcggttcggt ggcaatcaag attacggaac actcttggaa cgctgaccta tacaaactta
      661 tgggccattt cgcgtggtgg accgcctttg ttactaatgt caacgcgagc agctctgagg
      721 cattcctgat cggatgcaat tacctgggta aaccgcgaga acagatcgat ggctatgtta
      781 tgcacgctaa ctacatattc tggcgtaaca cgaacccgat ccaattatcc tcatattccc
      841 tgtttgacat gagcaaattc ccactaaaac tgcgcggtac agcagtgatg tctctgaaag
      901 aaggccagat taacgacatg attctgagtc tgctgtctaa agggagactg atcatccgtg
      961 agaataaccg tgtggtcatc tcatccgatg ttctggtaaa caatgagaac ctgtactttc
     1021 aaagcgagca gaagcttatt tccgaagaag atctctaacc ggcttatcgg tcagtttcac
     1081 ctgatttacg taaaaacccg cttcggcggg tttttgcttt tggaggggca gaaagatgaa
     1141 tgactgtcca cgacgctata cccaaaagaa attgagatcc tttttttctg cgcgtaatct
     1201 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1261 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1321 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1381 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1441 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1501 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1561 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1621 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1681 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1741 gggggcggag cctatggaaa ttgatcgggc acgtaagagg ttccaacttt caccataatg
     1801 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     1861 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     1921 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     1981 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     2041 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag
     2101 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     2161 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     2221 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     2281 ttgagaatat gtttttcgtt tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     2341 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     2401 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     2461 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     2521 cgtaatttga tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa
     2581 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggt
//
