LOCUS       sequence-287820-        1980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1980)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046842
FEATURES             Location/Qualifiers
     source          1..1980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             354..374
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             375..401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      518..1106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1007..1026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1212..1871
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1278..1297)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1942..1976
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg aaaatgtcag
      121 atgtaaagtg cacatcagta gtcttactct cagttttgca acaactcaga gtagaatcat
      181 catctaaatt gtgggctcaa tgtgtccagt tacacaatga cattctctta gctaaagata
      241 ctactgaagc ctttgaaaaa atggtttcac tactttctgt tttgctttcc atgcagggtg
      301 ctgtagacat aaacaagctt tgtgaagaaa tgctggacaa cagggcaacc ttagagaacc
      361 tgtactttca aagctacccg tatgacgttc ctgattatgc ctaaccggct tatcggtcag
      421 tttcacctga tttacgtaaa aacccgcttc ggcgggtttt tgcttttgga ggggcagaaa
      481 gatgaatgac tgtccacgac gctataccca aaagaaattg agatcctttt tttctgcgcg
      541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1081 cgtcaggggg gcggagccta tggaaattga tcgggcacgt aagaggttcc aactttcacc
     1141 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
     1201 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     1261 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     1321 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     1381 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
     1441 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     1501 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1561 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     1621 ggtttattga gaatatgttt ttcgtttcag ccaatccctg ggtgagtttc accagttttg
     1681 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     1741 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     1801 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     1861 gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc
     1921 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
//
