LOCUS       sequence-287821-        1863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1863)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046915
FEATURES             Location/Qualifiers
     source          1..1863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             237..257
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             258..284
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      401..989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     890..909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1095..1754
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1161..1180)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1825..1859
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg attgaattgt
      121 cactgatcga tttctatctt tgttttctcg cgttcctgct gtttctggtt ctgatcatgc
      181 tgattatctt ctggttctcc ttagagctgc aagaccataa tgaaacctgc cacgccgaga
      241 acctgtactt tcaaagctac ccgtatgacg ttcctgatta tgcctaaccg gcttatcggt
      301 cagtttcacc tgatttacgt aaaaacccgc ttcggcgggt ttttgctttt ggaggggcag
      361 aaagatgaat gactgtccac gacgctatac ccaaaagaaa ttgagatcct ttttttctgc
      421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      961 gctcgtcagg ggggcggagc ctatggaaat tgatcgggca cgtaagaggt tccaactttc
     1021 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     1081 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     1141 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     1201 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     1261 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
     1321 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     1381 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     1441 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     1501 aagggtttat tgagaatatg tttttcgttt cagccaatcc ctgggtgagt ttcaccagtt
     1561 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     1621 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1681 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     1741 agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga tccagtaatg
     1801 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     1861 ggt
//
