LOCUS       sequence-287833-        2607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2607)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046936
FEATURES             Location/Qualifiers
     source          1..2607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             981..1001
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1002..1028
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      1145..1733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1634..1653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1839..2498
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1905..1924)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2569..2603
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg tccgcggtga
      121 agcgtacgat taaaggcact caccattggc tgttacttac aatcctaacc agcctgctgg
      181 tcctcgttca aagtactcag tggtcgctgt tctttttctt gtatgagaac gccttcctgc
      241 cgtttgcaat ggggatcata gctatgtcag cgttcgctat gatgtttgtt aaacacaaac
      301 atgcattctt atgtctgttt ctgctgccaa gcctggctac cgtagcatac ttcaatatgg
      361 tttacatgcc ggcttcctgg gtgatgcgca ttatgacttg gctggatatg gtagacacct
      421 ctctgtctgg tttcaagctt aaagactgcg ttatgtatgc cagcgcggtg gttctgctga
      481 tcctgatgac cgctaggacc gtctacgatg atggtgctcg tcgcgtatgg actctaatga
      541 acgttctgac tcttgtatac aaagtctatt acggcaacgc actggaccag gcgatctcta
      601 tgtgggccct gatcatttct gttacatcca attacagtgg tgtagttacc acggtaatgt
      661 tccttgcccg tggcattgtg ttcatgtgcg tggaatattg ccctatcttc tttatcactg
      721 gtaacaccct gcaatgtatc atgttggttt actgctttct gggctacttt tgcacttgct
      781 atttcgggct gttctgttta ctcaaccgtt actttcgcct gaccctgggt gtctacgact
      841 acctggttag cacacaggaa tttcgctaca tgaacagcca gggactgctt cccccgaaaa
      901 attctattga tgctttcaaa ctgaacatca agttgttggg cgtgggtggc aaaccgtgca
      961 tcaaagtggc gacggttcag gagaacctgt actttcaaag ctacccgtat gacgttcctg
     1021 attatgccta accggcttat cggtcagttt cacctgattt acgtaaaaac ccgcttcggc
     1081 gggtttttgc ttttggaggg gcagaaagat gaatgactgt ccacgacgct atacccaaaa
     1141 gaaattgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1201 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1261 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1321 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1381 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1441 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1501 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1561 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1621 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1681 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaattgatcg
     1741 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     1801 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1861 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1921 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1981 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     2041 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     2101 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     2161 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     2221 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtttcagcca
     2281 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     2341 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     2401 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     2461 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     2521 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     2581 ggttgccgcc gggcgttttt tattggt
//
