LOCUS       sequence-287839-        2604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 nsp6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2604)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046892
FEATURES             Location/Qualifiers
     source          1..2604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             981..1001
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1002..1025
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     rep_origin      1142..1730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1631..1650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1836..2495
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1902..1921)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2566..2600
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg tccgcggtga
      121 agcgtacgat taaaggcact caccattggc tgttacttac aatcctaacc agcctgctgg
      181 tcctcgttca aagtactcag tggtcgctgt tctttttctt gtatgagaac gccttcctgc
      241 cgtttgcaat ggggatcata gctatgtcag cgttcgctat gatgtttgtt aaacacaaac
      301 atgcattctt atgtctgttt ctgctgccaa gcctggctac cgtagcatac ttcaatatgg
      361 tttacatgcc ggcttcctgg gtgatgcgca ttatgacttg gctggatatg gtagacacct
      421 ctctgtctgg tttcaagctt aaagactgcg ttatgtatgc cagcgcggtg gttctgctga
      481 tcctgatgac cgctaggacc gtctacgatg atggtgctcg tcgcgtatgg actctaatga
      541 acgttctgac tcttgtatac aaagtctatt acggcaacgc actggaccag gcgatctcta
      601 tgtgggccct gatcatttct gttacatcca attacagtgg tgtagttacc acggtaatgt
      661 tccttgcccg tggcattgtg ttcatgtgcg tggaatattg ccctatcttc tttatcactg
      721 gtaacaccct gcaatgtatc atgttggttt actgctttct gggctacttt tgcacttgct
      781 atttcgggct gttctgttta ctcaaccgtt actttcgcct gaccctgggt gtctacgact
      841 acctggttag cacacaggaa tttcgctaca tgaacagcca gggactgctt cccccgaaaa
      901 attctattga tgctttcaaa ctgaacatca agttgttggg cgtgggtggc aaaccgtgca
      961 tcaaagtggc gacggttcag gagaacctgt actttcaaag ctggagccat ccgcagtttg
     1021 aaaaataacc ggcttatcgg tcagtttcac ctgatttacg taaaaacccg cttcggcggg
     1081 tttttgcttt tggaggggca gaaagatgaa tgactgtcca cgacgctata cccaaaagaa
     1141 attgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1261 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa ttgatcgggc
     1741 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     1801 agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     1861 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     1921 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     1981 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     2041 ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     2101 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     2161 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     2221 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtt tcagccaatc
     2281 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     2341 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     2401 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     2461 aacagtactg cgatgagtgg cagggcgggg cgtaatttga tatcgagctc gcttggactc
     2521 ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt
     2581 tgccgccggg cgttttttat tggt
//
