LOCUS       sequence-287854-        2097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2097)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046732
FEATURES             Location/Qualifiers
     source          1..2097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    39..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             471..491
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             492..518
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      635..1223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1124..1143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1329..1988
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1395..1414)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2059..2093
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
       61 tcccctctag aaataatttt gtttaacttt aagaaggaga ctcttctatg aagatcatac
      121 tattcctcgc cttgatcacc ctggcaacgt gtgaactgta tcactaccaa gagtgcgttc
      181 gcggtactac tgtcctgctt aaagaaccat gcagctcagg gacctacgaa ggcaactctc
      241 cgtttcatcc tctggcggac aataaattcg ctttaacatg cttttcgact cagttcgctt
      301 tcgcttgtcc cgatggcgta aaacacgtgt accagctgcg tgcgcgttcc gtttctccga
      361 aactgtttat tcgacaggag gaagtgcagg aactgtattc cccgattttc ctgatcgtag
      421 ccgcaatcgt tttcatcacc ctgtgcttta cccttaagcg caaaactgag gagaacctgt
      481 actttcaaag ctacccgtat gacgttcctg attatgccta accggcttat cggtcagttt
      541 cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg gcagaaagat
      601 gaatgactgt ccacgacgct atacccaaaa gaaattgaga tccttttttt ctgcgcgtaa
      661 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      721 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      781 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      841 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      901 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      961 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1021 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1081 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1141 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1201 caggggggcg gagcctatgg aaattgatcg ggcacgtaag aggttccaac tttcaccata
     1261 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     1321 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
     1381 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     1441 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     1501 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
     1561 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     1621 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     1681 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     1741 ttattgagaa tatgtttttc gtttcagcca atccctgggt gagtttcacc agttttgatt
     1801 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     1861 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
     1921 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1981 gggcgtaatt tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca
     2041 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggt
//
