LOCUS       sequence-28789-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S,
            Callaway EM
  TITLE     Short Promoters in Viral Vectors Drive Selective Expression in 
            Mammalian Inhibitory Neurons, but do not Restrict Activity to 
            Specific Inhibitory Cell-Types.
  JOURNAL   Front Neural Circuits. 2009 Nov 9;3:19. doi: 
            10.3389/neuro.04.019.2009. eCollection 2009.
  PUBMED    19949461
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2009-11-9"; volume: "3:19. doi"; pages: " 
            10.3389/neuro.04.019.2009. eCollection 2009"
COMMENT     Sequence Label: pAAV-mA93-RFP
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..673)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LF"
     primer_bind     complement(58..81)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(256..275)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     473..493
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     525..543
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      670..679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggaacaggtg gtgncggccc tcggcgcgct cgtactgctc cacgatggtg tagtcctcgt
       61 tgtgggaggt gatgtccagc ttggagtcca cgtagtagta gccgggcagc tgcacgggct
      121 tcttggccat gtagatggac ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca
      181 gggccttgtg gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg
      241 cctcccagcc catagtcttc ttctgcatta cggggccgtc ggaggggaag ttcacgccga
      301 tgaacttcac cttgtagatg aaggagccgt cctgcaggga ggagtcctgg gtcacggtca
      361 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
      421 gcttcttgta gtcggggatg tcggcggggt gcttcacgta caccttggag ccgtactgga
      481 actgggggga caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct
      541 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
      601 ggccgttcac ggagccctcc atgcgcacct tgaagcgcat gaactccttg atgacgtcct
      661 cggaggaggc catggtggcg accggtggcc gaggcggcca gatcttcgaa ttcacgtgct
      721 ttgncaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag
      781 ataagaggta tgaacatgat tagcaaaagg gcctagctt
//
