LOCUS       sequence-287984-       12781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of BE3RA in mammalian 
            cells (codon optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY,
            Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, 
            Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow 
            LE
  TITLE     Optimized base editors enable efficient editing in cells, organoids 
            and mice.
  JOURNAL   Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
  PUBMED    29969439
REFERENCE   2  (bases 1 to 12781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194."
COMMENT     Sequence Label: pLenti-BE3RA-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..12781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(380..484)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(510..965)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(647..668)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     859..878
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1092..1114
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1106..1123
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1107..1123
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1253..1315
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1736..2115
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2117..2315
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2333..2513
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(2575..3163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2655..2674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3334..4125,4126..4194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4195..4299)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4374..4393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4565..4944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4946..5144
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5162..5342
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5389..5514
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6011..6244
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6429..6473
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6771..6888
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7006..7217
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             7253..7939
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(7988..8011,8012..8014,8015..12088)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7988..12088
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             12101..12349
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             12362..12382
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(12444..12467)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
       61 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      481 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
      541 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
      601 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
      661 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
      721 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
      781 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
      841 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
      901 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
      961 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     1021 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     1081 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat
     1141 tcacgttttt gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt
     1201 gatggagcag atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa
     1261 agataatcat attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt
     1321 tatcggtctg tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat
     1381 ttacacttac atactaataa taaattcaac aaacaattta tttatgttta tttatttatt
     1441 aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc
     1501 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     1561 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     1621 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
     1681 tgctctttga gcctgcagac acctgggggg atacggggaa aaggcctcca cggccgacat
     1741 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     1801 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     1861 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     1921 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1981 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2041 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2101 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2161 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2221 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2281 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
     2341 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     2401 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     2461 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg
     2521 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2581 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2641 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2701 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2761 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2821 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2881 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2941 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3001 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3061 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3121 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3181 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3241 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3301 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3361 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3421 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3481 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3541 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3601 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3661 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3721 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3781 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3841 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3901 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3961 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     4021 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     4081 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     4141 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4201 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4261 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4321 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     4381 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     4441 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     4501 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     4561 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4621 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4681 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4741 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4801 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4861 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4921 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     4981 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     5041 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     5101 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
     5161 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5221 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5281 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5341 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     5401 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     5461 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     5521 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     5581 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     5641 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     5701 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     5761 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     5821 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     5881 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     5941 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     6001 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6061 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6121 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     6181 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     6241 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     6301 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     6361 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     6421 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     6481 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     6541 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     6601 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     6661 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     6721 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     6781 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     6841 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     6901 agggacagca gagatccagt ttggttagta ccgggcccgg gtcgactagt gatcagtgtg
     6961 agggagtgta aagctggttt tcgagtggct ccagtgcccg tcagtgggca gagcgcacat
     7021 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     7081 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     7141 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     7201 ttgccgccag aacacaggtg tcgtgacgcg gctaactcga acgctagcca ccatgagctc
     7261 agagactggc ccagtggctg tggaccccac attgagacgg cggatcgagc cccatgagtt
     7321 tgaggtattc ttcgatccga gagagctccg caaggagacc tgcctgcttt acgaaattaa
     7381 ttgggggggc cggcactcca tttggcgaca tacatcacag aacactaaca agcacgtcga
     7441 agtcaacttc atcgagaagt tcacgacaga aagatatttc tgtccgaaca caaggtgcag
     7501 cattacctgg tttctcagct ggagcccatg cggcgaatgt agtagggcca tcactgaatt
     7561 cctgtcaagg tatccccacg tcactctgtt tatttacatc gcaaggctgt accaccacgc
     7621 tgacccccgc aatcgacaag gcctgcggga tttgatctct tcaggtgtga ctatccaaat
     7681 tatgactgag caggagtcag gatactgctg gagaaacttt gtgaattata gcccgagtaa
     7741 tgaagcccac tggcctaggt atccccatct gtgggtacga ctgtacgttc ttgaactgta
     7801 ctgcatcata ctgggcctgc ctccttgtct caacattctg agaaggaagc agccacagct
     7861 gacattcttt accatcgctc ttcagtcttg tcattaccag cgactgcccc cacacattct
     7921 ctgggccacc gggttgaaaa gcggcagcga gactcccggg acctcagagt ccgccacacc
     7981 cgaaagtgac aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc
     8041 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     8101 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     8161 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     8221 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     8281 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     8341 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     8401 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     8461 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     8521 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     8581 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     8641 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     8701 caacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     8761 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     8821 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     8881 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     8941 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     9001 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     9061 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     9121 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     9181 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     9241 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     9301 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     9361 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     9421 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     9481 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     9541 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     9601 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     9661 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     9721 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     9781 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     9841 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     9901 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     9961 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
    10021 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
    10081 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
    10141 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
    10201 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
    10261 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
    10321 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
    10381 cccagtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
    10441 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
    10501 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
    10561 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
    10621 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
    10681 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
    10741 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
    10801 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
    10861 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
    10921 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
    10981 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
    11041 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
    11101 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
    11161 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
    11221 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
    11281 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
    11341 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
    11401 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
    11461 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
    11521 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
    11581 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
    11641 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
    11701 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
    11761 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
    11821 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
    11881 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
    11941 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
    12001 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
    12061 gatcgacctg tctcagctgg gaggcgattc aggcggatct actaatctgt cagatattat
    12121 tgaaaaggag accggtaagc aactggttat ccaggaatcc atcctcatgc tcccagagga
    12181 ggtggaagaa gtcattggga acaagccgga aagcgatata ctcgtgcaca ccgcctacga
    12241 cgagagcacc gacgagaatg tcatgcttct gactagcgac gcccctgaat acaagccttg
    12301 ggctctggtc atacaggata gcaacggtga gaacaagatt aagatgctct ctggtggttc
    12361 tcccaagaag aagaggaaag tctaactagg aatcccgagg cctctaccgg gtaggggagg
    12421 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
    12481 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
    12541 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
    12601 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
    12661 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
    12721 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
    12781 g
//
