LOCUS       sequence-287990-       10957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF13B 442-1843.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF13B tail 442-1843
FEATURES             Location/Qualifiers
     source          1..10957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     727..749
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(817..819,820..822,823..1530)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(862..883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1123..1142)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1470..1491
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5885..6019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5922..5941)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5976..5995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6050..6066)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6050..6066)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6063..6085)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6074..6090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6098..6104,6105..6122,6123..6128))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6143..6164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6281..6298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6452..7040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6532..6551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7211..8002,8003..8071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7834..7853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8072..8176)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8244..8262
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8300..8322)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8422..8441
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8635..8657
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8649..8666
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8650..8666
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10467..10741
                     /label=chicken beta-actin promoter
ORIGIN
        1 ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc
       61 cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag
      121 tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg
      181 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc
      241 ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
      301 cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
      361 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
      421 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
      481 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
      541 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
      601 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
      661 tccatctcca gcctcggggc tgtccgcagg gggacggctg ccttcggggg ggacggggca
      721 gggcggggtt cggcttctgg cgtgtgaccg gcggggttta tatcttccct tctctgttcc
      781 tccgcagccc ccaagcttgg cgcgcctagg cctaccatgg tgagcaaggg cggggagctg
      841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      901 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1021 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1081 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1141 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1201 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1261 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1321 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1381 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1441 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1501 gggatcactc tcggcatgga cgagctgtac ggcggaggat ccggaggcgg ctctggcgga
     1561 gggctgtaca agggcggagg atccggaggc ggctctggcg gagggaaggt tggggatgac
     1621 aagtgcttcc tggttaacct gaatgctgac ccagctctga atgaactcct ggtgtactac
     1681 ttaaaggagc acacgctgat agggtcggcc aattctcagg acatccagct ctgcgggatg
     1741 ggcatcctcc ctgagcacgg catcatagac atcacgcccg agggccaggt tgtgctgact
     1801 cctcagaaga acaccagaac atttgtcaat gggtcttctg tctccagccc catccagctg
     1861 caccatgggg acaggatact gtggggaaac aaccatttct tcagactcaa tctgccaaaa
     1921 aagaaaaaga aagcagagcg agaggacgag gaccgtgagg catccctgaa aaacgacagt
     1981 agttctgagc agctggatgc cgacggcgac tcctccagtg aggtgtccag cgagatcaac
     2041 ttcaactttg agtatgcaca gatggaggtg accatgaagg ctctgggcag caatgaccca
     2101 atgcagtcca tactgagcag cctagaacag cagcatgagg aggagaagcg gtctgctctg
     2161 gagcgccaga ggctcatgta cgaacacgaa ctggaacagc ttcgaagaag gctgtctcct
     2221 gagaggcagg actgccgcgg tgtggaccgg ctctccttcc actcgcccag tgcccagcag
     2281 cgcttgaaac agtgggctga agagagagaa gcaacattga ataacagctt gatgaggctg
     2341 agggagcaga ttgtaaaggc caacctgctg gttcgggaag ctagttacat cgcagaagag
     2401 ttggataaaa gaaccgagta taaagttacc ctccagattc caacctccag tctggatgcc
     2461 aataggaagc gaggctctct cctcagtgaa cctgccatcc aggtgagaag aaagggcaaa
     2521 ggaaagcaga tttggtcttt ggaaaagctg gaaaacaggc ttttggatat gagagacctc
     2581 taccaggagt ggaaggagtg tgaagaagac agcccggtta gccggtccta cttcaagcgt
     2641 gctgacccat tctatgatga gcaggaaaac catagtctca tcggtgtggc caatgtcttc
     2701 ctggagaccc tgttttatga cgtgaagtta cagtatgctg tgccgatcat caaccagaag
     2761 ggagaggtgg caggccggct gcatgtggag gtgatgcgac tcagtggggc tattggggag
     2821 aggattgcag gaggagacga tcctacagag gtcagctctg aaaaggaagc ccaggagaac
     2881 aggctggtct gcatggttaa aatactccaa gccacagggt tgccgcagca tctgtgtcac
     2941 tttgtcttct gcaaatatga cttctgggat cagcaggagc cagtgaccgt tgcccctgaa
     3001 gtggatacgt cttcgtctcc caccagcaag cagccacagt gtatggttgt gttcgatcac
     3061 tgcagtgagt tcagtgttaa catcacagaa gacttcattg aatatctctc agaaggggcc
     3121 ctagccattg aagtatatgg acataaaatg aatgatcccc ggaaaaaccc agccctgtgg
     3181 gacttgggga tcatccaagc aaaaacaagg agtcttcggg acaggtggag tgaagtcacc
     3241 agaaagttgg aattctgggt tcagatcttg gaacagaatg agaatggtga ttactgtccg
     3301 gtggaagtaa ttgctgcaaa agatgtcccc acaggaggga tcttccagct ccgacagggg
     3361 cagtctcggc gagttcaagt tgaagtgaag tctgtgcagg agtcaggaac tttaccactg
     3421 atggaagagt gcatattgtc agttggcatt gggtgtgtga aagttaggcc actccggtcc
     3481 cctaagatcc acgagaatgt ccatgaggaa gaggaggaca tggacagcta ccaggatagg
     3541 gatttagagc ggcttcgtag aaaatggctc aatgcattaa ccaaacgtca agaatacttg
     3601 gatcaacaac tgcagaagct tgtcagtaaa catgacaaaa ctgaggatga tgctgaccga
     3661 gaggcacagc tcctggagat gcggctgacg ctcacagagg aaagaaatgc agtgatggtg
     3721 ccttctgctg ggagtggcat tccaggggcc cctgcagaat ggaccccagt gcctgggatg
     3781 gagacccaca ttcctgttat atttcttgac ttaaatgcgg atgatttcag ctctcaggat
     3841 aatctcgatg acccagaagc aggatgggat gctaccctga ctggggaaga ggaggaggag
     3901 ttctttgagc tgcagattgt gaaacaacac gatggagagg tgaaggcaga ggcctcctgg
     3961 gactctgcag tgcacagctg tcctcaactc agtaagggca cacctgctga cgagcgagtg
     4021 ttcctcatcc tgcgagtgac tgtccagctc agccatcctg ccgacatgca gctggtccta
     4081 cgcaagagga tttgtgtcca tgttcatggg cgccagggct ttgctcaaag tctcctcaaa
     4141 aagatgtctc accggagctc tattcctggc tgtggagtga cctttgaaat cgtctccaat
     4201 attccagagg atgcccaggg agtggaggag cgagaggcac tggcaaggat ggcagctaac
     4261 gtggaaaacc cagcatctgc tgactccgag gcctatattg agaagtactt gaggagtgtg
     4321 ctggccgtgg aaaacctcct gactttagat cgcctgcgtc aggaagtggc cgtgaaggag
     4381 cagctgacgg ggaaggggaa gctgagccga aggagtatca gctcccccag catgaacaga
     4441 ttgtcaggaa gccggcagga gcttagccca tcccacagtc taggcagcaa caagggccgg
     4501 tgggaaagtc aacaggatgt gtcccaaaca ctagtttcca gaggaatagc tccaggccct
     4561 ccagctctct ctgtttcgcc ccaaaataac cagtctcctg atcctggact gggaggcgtg
     4621 gcagcctctt acttgaaccc agtgaagtcc ctggtgccac agatgccaaa gctgctgaag
     4681 tctctcttcc ctgttcggga tgacagaagg gggagacact catctcccct tgcacaccag
     4741 gctcaacaag gcagccctgg gcctagtggg gctttggaat ccatggtgaa gatggcggcc
     4801 cctgcagtaa agatctgtga caaacctgtc aaagtgtctt cccctccatc tcccatggtg
     4861 gttactcagc ccccagaagg acaggatgga ccacccagcc ctttgagtga agcttcaagt
     4921 ggctacttct ctcacagtgt ctcttctgcc accctgtcag aaacactcac cttaggcctg
     4981 gacaccacag gcctcggcag ccagacgcct ggttcccccc ctgccctgtg tcaggtcacc
     5041 ccagagcctg agctggcttt cctctcctgc acactaagcc atcctcctgc cccagaggag
     5101 gcccacgtac ctgcagctcc tactcaaagc acagagctgg aggttcccag agcacctctt
     5161 ctttctgaac ccgcgtctgc tgtacccacg tccccattca gaatccggaa agtgcggacc
     5221 tcagagctta agtccttcac aggcatgctg ggtggtgcct cctccggagc agaggaggac
     5281 cccctggctt cagaagaccc cagcaatgct aggggacaga ctctggggag gctagaagta
     5341 acatctgact ctgaggatgc cagcgaagtc cctgagtggc tcagggaggg agagtatgtt
     5401 gttgtgggca ccaacaagac gggcattgta cggtatattg gaccaactga tttccaggag
     5461 ggaacatgga ttggtgtgga gctggatctg cctgcaggaa agaatgatgg ctccattggt
     5521 gggaaacagt atttcaggtg caaccctggc tatgggctgc tggtgaggcc cagccgggtc
     5581 cgcagggctg tgggcactgg gcgcaggcgc agttcagggc tccagcctca gggagcccca
     5641 gaggtccgca gaagtgctac catctcgggc tctgccacca acctggcttc actgacagct
     5701 gcactggcca agggagacag gagttacaag aatcctgaga accggaagtc ctgggccagc
     5761 taagtctaga taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc
     5821 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5881 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5941 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6001 atcttatcat gtctggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt
     6061 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6121 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6181 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6241 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8221 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     8281 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     8341 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     8401 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     8461 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     8521 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     8581 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     8641 cacgacgttg taaaacgacg gccagtgcca agttgggatc tttgcattgg cccacggctc
     8701 tcaggatggg gatgctcccc ttcagcaccc ggttcccctt ggaaactgat ggtcctggct
     8761 ctgtggcatg gcagtggcac tgtgaggagc cccctaccag cagcacacag tggggttggc
     8821 actgccacgc tccggatgcc gcgctctgat ccaaccccat aatcaaggga acccgaattg
     8881 ccccatcatt gcccccacca cccccatcct gccgggccct cacaccccac gctgccttct
     8941 ggtgacattc cccagcccaa acccacggct tcatggctac cgcggggcat ttcccattgc
     9001 cgccccatta tcagctctgc acacctcccg ctgtacccat gcctcgtggc tgcccttctt
     9061 tgacgtataa tcttctaatt aatacccggc cttgtcaaag tggagcacaa acgttaatta
     9121 attccccagc aggcaggtaa ttaacagtgt gactcccttt ttgctgcgag tggggctgat
     9181 acagagagat gtggcactat ggagcccacg gggtcctggc actgggtgcc cacggaggtc
     9241 cccatgtgct gcagtgtcac cgcctccgag gagacagtat tgtccctgcg gtgtccctgc
     9301 agctcagctc tgtccacagg gccacctcca gtttggaggg gacacaatgc agccccgatg
     9361 caacccatcc tcgcagcatc ccagggacaa agaccccact gcaagaccgc acacagggct
     9421 gggtcccgct cccctaatat ctacagtgct tcttgcatgg ccccttaatc aatgcagtta
     9481 atcagcatgc gctcatgcac cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca
     9541 acaccgcgca ccgccccggc ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa
     9601 tattgcccaa atgtaggcaa aggcattcgg ctgccttgac ctccgccggg ccgggccctg
     9661 cctgactcag ctccttactc agcgctcgct tcctccctcc ggctgccacc gccgcagcgc
     9721 acaccctgac aaagagtggc ccttaacggg ctctgaggtg cacccagcag tgcactcagc
     9781 agtccaaggg ccggcctgga ggtttgcacc gctacgtgct gacattagca ttgaacttgg
     9841 ccctgggtag tgctgcaggc cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc
     9901 cggtgcccct tcccctccct tgcatcccag caggctgcac cccagcacca ggcccgtgca
     9961 tgcatgctcc tggtgttatt gcagcctggt gcatgcatgc gtcttgatgg tgcagcgctg
    10021 tgcatgcatc ctccttggtg tgtagcagtc tagtgcatgc atacccctcg gtgttattgc
    10081 tgctctgtgc acgcacgctc attgtatcac ttcatcccag tgcatgcact cacactggag
    10141 cgattgctgc tcggtgcacg cacactcatt gtatcactgc agctcagtgg ctgcacgcac
    10201 accggtgtta ttgctgctcg gtgcgtgcat gcacatcagt gtcgctgcag ctcagtgcat
    10261 gcacgctcat tgcccatcgc tatccctgcc tctcctgctg gcgctccccg ggaggtgact
    10321 tcaaggggac cgcaggacca cctcgggggt ggggggaggg ctgcacacgc ggaccccgct
    10381 ccccctcccc aacaaagcac tgtggaatca aaaagggggg aggggggatg gaggggcgcg
    10441 tcacaccccc gccccacacc ctcacctcga ggtgagcccc acgttctgct tcactctccc
    10501 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
    10561 agcgatgggg gcgggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
    10621 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
    10681 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
    10741 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
    10801 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
    10861 tccgggctgt aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga
    10921 aagccttaaa gggctccggg agggcccttt gtgcggg
//
