LOCUS       sequence-288020-       14788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS 
            Reagent Program Cat #11051) and D185A mutation in reverse 
            transcriptase (RT D185A)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, 
            Sundquist WI
  TITLE     Reconstitution and visualization of HIV-1 capsid-dependent 
            replication and integration in vitro.
  JOURNAL   Science. 2020 Oct 9;370(6513). pii: 370/6513/eabc8420. doi: 
            10.1126/science.abc8420.
  PUBMED    33033190
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-10-9"; volume: "370(6513). pii: 370/6513/eabc8420. doi";
            pages: " 10.1126/science.abc8420"
COMMENT     Sequence Label: SG3-Delta-ENV RT D185A
FEATURES             Location/Qualifiers
     source          1..14788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1933..2388
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2020..2039)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2230..2251
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2514..2536
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2528..2545
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2529..2545
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             4688..4944
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4991..5116
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9091..9208
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     RBS             10998..11009
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    12091..12324
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12509..12553
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     LTR             13785..14041
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     RBS             14242..14250
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(14530..14549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14531..14549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14568..14584)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14568..14584)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14581..14603)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14592..14608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14616..14622,14623..14640,14641..14646))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14661..14682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     1981 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     2041 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     2101 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     2161 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2221 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     2281 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2341 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
     2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     2461 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     2521 acgacgttgt aaaacgacgg ccagtgaatt ccttctatgg aatacttttt aaatatatag
     2581 tctgataata tataactgac acgttttctg ttgtttcata atggttgatt ttctcagggc
     2641 tacgtttctc tattttttcc tgttctcaaa tcttaactct ccatctctgt cctcaagtgg
     2701 taagtgatct tgtgtcttat tttgaaatta aagtcatcaa caaattattt caactcatag
     2761 atttatagtt gtggaagact tactattcaa atggaattag aacgttttgg gggataggat
     2821 gcagtagttc ttggtagtcc aagactctta ataaaacaac tagaaaagcc aggtaattta
     2881 caaaatttat atatttagcg atagagaaca atgggtgtaa aaatgattaa agaaagcaaa
     2941 attttgcacc ctgggggaat acctttcaca agtgagctga gaggtctgca gctgatcatt
     3001 gtttttcctg ggtacagagt agaggaggcc tttgggctgc agcctgagtg gcagattgga
     3061 aatatgaggg gcctttaaac acagagaacg ccctcagtat attcctaaat tctgtagttc
     3121 agctgggcgg aagagataaa ccaaaatctc agaaaaggag attgggatat tttctagtcc
     3181 tatggtgttg agataaagat tctctagggg aagcgacttg agaacaatag gtgagagttg
     3241 aaagcgtttg aggaagtggt gaatacacac tgatgatttt caacacaccc agtttcttcc
     3301 cccgtagaca tttgccaaat tctgaagctg cttaggctag agagctaaac cagaagtctc
     3361 ttaaaaacaa agcaattctt atagtatcta ggtacctaga agataacgat ctgctagctt
     3421 ttgaaatgaa ggctctgcag agtctcatcc taggaacagg gacaaaccag aggtagtctc
     3481 cttcatagca aaactgctac tcagcagtgt tctatgattc tggcattata gaggaagtag
     3541 taaaagtata tgtatgaaaa ttgtgttaag tgaaggatga ataagaaaat atctagggta
     3601 accactaaaa gaaaagaatg tgtaacaaat gaatacagaa aaaaattgga tatagaatat
     3661 gtgataaatt cagaagaaga caagaaagaa aatataaaac aagcaggcaa ctaaattagt
     3721 aattatgtta catggaaatg ggctaaatat ttcaagtaaa tggcaaaaat catcagactg
     3781 gacaaataat aattacctaa atataagtac ctagaaagat tgagagaaaa tgatgcgaaa
     3841 aggtatacca tccaaatgct aactgaagga aataagtgtg gctataccaa tcaggcaaag
     3901 gagactttaa tacaataaac agtagtagaa aaagagaact acattttata gtgtaagggg
     3961 attgatccac cagagttacc acaatccttt atgtgtaccc agtaaaatag cccaaacatc
     4021 tataaaccaa aaattttatt ttgaactaaa agaaacagac aaatatgccc tcacaatgga
     4081 tgcacatata taggacatac atacatcaca attagcaggc ttgatctaat tgtcatacgt
     4141 agaacattga aactattaat ggtggtatta accatctttt cccttgcaca tggaaattgt
     4201 actagaattg accataagct agggccatga acaaagcctc agcaaatatc attgatttta
     4261 ataattgaat atgttgtttg tattatagta aataaaaata actagaaaat tggaagggct
     4321 aattatctcc caaagaagac aagatatcct tgatctgtgg acctaccaca cacaaggcta
     4381 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     4441 atggtgtttc aagctagtac cagttgatcc agagaagata gaagaggcca atgaaggaga
     4501 aaacaacagc ttgctacacc ctatgagcca gcatgggatg gatgacccgg agagagaagt
     4561 gctagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     4621 ggagtactac aagaaccgat gacatcgcgc tttctacaag ggactttccg ctggggactt
     4681 tccaggggag gcgtggcctg ggcgggaccg gggagtggcg agccctcaga tgctgcatat
     4741 aagcagctgc ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4801 tctctggcta gctagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4861 aagtagtgtg tgcccgtctg ttgtgtgact ctggtagcta gagatccctc ataccctttt
     4921 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gaccggaaag cgaaagagaa
     4981 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     5041 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     5101 tgggtgcgag agcgtcagta ttgagcgggg gaaaattgga taagtgggag aaaattcggt
     5161 taaggccagg gggaaagaaa aaatataaat taaaacatct agtatgggca agcagggagc
     5221 tagaacgatt cgcagttaat cccggcctgt tagaaacagc agaaggctgt agacaaatac
     5281 tgggacagct acaaccgtcc cttcagacag gatcagaaga acttaaatca ttatataata
     5341 caatagcagt cctctattgt gtgcatcaaa tgatagatgt aaaagacacc aaggaagctt
     5401 tagagaagat agaggaagag caaaacaaca gtaagaaaaa agcacagcaa gcagcagctg
     5461 acacaggaaa cagcagccag gtcagccgaa attaccctat agtgcagaac atccaggggc
     5521 aaatggtaca tcaggccata tcacccagaa ctttaaatgc atgggtaaaa gtagtagaag
     5581 agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa ggagccaccc
     5641 cacaagattt aaacaccatg ctaaacacag tggggggaca tcaagcagct atgcaaatgt
     5701 taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca gtgcaagcag
     5761 ggcctgttgc accaggccag atgagagaac caaggggaag tgacatagca ggaactacta
     5821 gtacccttca ggaacaaata ggatggatga caaataatcc acctatccca gtaggagaaa
     5881 tctataaaag atggataatc ctgggattaa ataaaatagt aaggatgtat agtccttcta
     5941 gcattctgga cataagacaa ggaccaaagg aatcctttag agactatgta gacaggttct
     6001 ataaaacttt aagagccgag caagcttcac aggaggtaaa aaattggatg acagaaacct
     6061 tgttggtcca aaatgcgaac ccagattgta agaccatttt aaaagcattg ggaccaggag
     6121 ctacactaga ggaaatgatg acagcatgtc agggagtggg aggacccggc cacaaagcaa
     6181 gagttttggc ggaagcaatg agccaagtaa caaattcagc caccataatg atgcagagag
     6241 gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa gaagggcata
     6301 tagccagaaa ttgcaaggcc cctaggaaaa ggggctgctg gaaatgtgga aaggaaggac
     6361 accaaatgaa agattgtaca gagagacagg ctaatttttt agggaagatc tggccttccc
     6421 acaagggaag gccagggaat tttcctcaga gcagactaga gccaacagct ccaccagaag
     6481 agagcttcag gtttggggag gagacaacaa ctccccctca gaagcaggag cgggaagaca
     6541 aggaaatgta tcccttagct tccctcagat cactctttgg caacgacccc tcgtcacaat
     6601 aaagataggg gggcaactaa aggaggctct attagataca ggagcagatg atacagtatt
     6661 agaagaaatg aatttgccag ggagatggaa gccaaaaatg atagggggaa ttggaggttt
     6721 tatcaaagta agacagtatg atcagatacc catagaaatc tgtggacata aagctatagg
     6781 tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt tgactcagat
     6841 tggttgcact ctaaattttc ccattagtcc tattgaaact gtaccagtaa aattaaagcc
     6901 aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa taaaagcatt
     6961 agtagaaatt tgtacagaaa tggaaaagga agggaagatt tcaaaaattg ggcctgaaaa
     7021 tccatacaat actccagtat ttgctataaa gaaaaaagac agtactaaat ggagaaaatt
     7081 agtagatttc agagaactta ataagaggac tcaagacttc tgggaagttc aattaggaat
     7141 accacatccc gctggattaa aaaagaaaaa atcagtaaca gtactagatg tgggtgatgc
     7201 atatttctca gttcccttag ataaagactt caggaaatat actgcattta ccatacctag
     7261 tataaacaat gagacaccag ggattagata tcagtacaat gtgctcccac agggatggaa
     7321 aggatcacca gcaatattcc aaagtagcat gacaaaaatc ttagagcctt ttagaaagca
     7381 aaatccagac atagttatct atcagtacat ggctgatttg tatgtaggat ctgacttaga
     7441 aatagggcag catagaacaa aaatagagga actgagacaa catctgttaa ggtggggatt
     7501 taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg gttatgaact
     7561 ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaagaca gctggactgt
     7621 caatgacata cagaagttag tgggaaaatt gaattgggca agtcagattt actcagggat
     7681 caaagtgaag cagttatgta aactccttag gggaaccaaa gcactaacag aagtagtaac
     7741 actaacagaa gaagcagagc tagaactggc agaaaacagg gaaattctaa aagaaccagt
     7801 acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga aacaggggca
     7861 aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa caggaaaata
     7921 tgcaagaacg aggggtgccc acactaatga tgtaaaacaa ttaacagagg cagtgcaaaa
     7981 aataaccaca gaatgcataa taatatgggg aaaaactcct aaatttagac tgcccataca
     8041 aaaagaaaca tgggaaacat ggtggacaga gtattggcaa gccacctgga ttcctgaatg
     8101 ggagtttgtc aatacccctc ccttagtgaa attatggtac cagttagaga aagagcccat
     8161 agaaggcgca gaaactttct atgtagatgg agcagctaac agggagacta aattaggaaa
     8221 agcaggatat gttactaaca aaggaagaca aaaagttgtc accctaactg acacaacaaa
     8281 tcagaagact gagttagaag caattcatct agctttgcag gattctggat tagaagtaaa
     8341 catagtaaca gactcacaat atgcattagg aatcattcaa gcacaaccag ataaaagtga
     8401 atcagaatta gtcagtcaaa taatagagca gttaataaaa aaggaaaagg tctacctggc
     8461 atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat tagtcagtgc
     8521 tggggtcagg aaagtactat ttttagatgg aatagataag gcccaagaag aacatgagaa
     8581 atatcacagt aattggagag ccatggctag tgattttaac ttaccacctg tagtagcaaa
     8641 agaaatagta gccagctgtg ataaatgtca gctaaaagga gaagccatgc atggacaagt
     8701 agactgtagt ccaggaatat ggcaactaga ttgcacacat ctagaaggaa aaattatcct
     8761 ggtggcggtt catgtagcca gtggatatat agaagcagaa gttattccag cagagacagg
     8821 gcaggaaaca gcatactttc tcttaaaatt agcaggaaga tggccagtaa aaacaataca
     8881 tacagacaat ggcagcaatt tcaccagtac cacggttaag gccgcctgtt ggtgggcagg
     8941 gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtag tagaatctat
     9001 gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac atcttaaaac
     9061 agcagtacaa atggcagtat ttatccacaa ttttaaaaga aaagggggga ttggggggta
     9121 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaactaca
     9181 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacaaca aagatccact
     9241 ttggaaagga ccagcaaagc ttctctggaa aggtgaaggg gcagtagtaa tacaagataa
     9301 tagtgacata aaagtagtgc caagaagaaa agcaaagatc attagagatt atggaaaaca
     9361 gatggcaggt gatgattgtg tggcaagtag acaggatgag gattagaaca tggataagtt
     9421 tagtaaaaca ccatatgtat atttcaagga aagcaaaggg atggttttat agacatcact
     9481 atgaaagcac tcacccaaaa ataagttcag aagtacacat cccactaggg gatgctagat
     9541 tggtaataac aacatattgg ggtctgcata caggagaaag agattggcat ttgggtcatg
     9601 gagtctccgt agaatggagg aaaaagagat atagcacaca agtagaccct gacctagcag
     9661 accaactaat tcatctgtat tactttgatt gtttttcaga atctgccata agaaatgcca
     9721 tattaggaca tatagttagt cctaggtgtg aatatcaagc aggacataac aaggtaggat
     9781 ctctacagta cctagcacta gcagcattaa taacaccaaa aaggataaag ccacctttgc
     9841 ctagtgttac aaaactaaca gaggatagat ggaacaagcc ccagaagacc aagggccaca
     9901 gagggagcca tacaatgaat ggacactaga gcttttagaa gaacttaaga atgaagctgt
     9961 tagacatttt cctaggatat ggctccatgg cttagggcaa tatatctatg aaacttatgg
    10021 ggatacttgg gcaggagtgg aagccctagt aagaactctg caacaactgc tgtttactct
    10081 tttcagaatt gggtgtcgac atagcagaat aggcattact caacgaagaa gagcaagaaa
    10141 tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagccag cctaaaactg
    10201 cttgtaccaa atgctattgt aaaaagtgtt gcttacattg ccaagtttgt ttcatgacaa
    10261 aaggcttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga gctcctcaag
    10321 acagtcaggc tcatcaagct tctctatcaa agcagtaagt agtgcatgta atgcaaccta
    10381 tacaaatagc agcaatagta gcattagtag tggtaggaat aatagcaata gttgtgtggt
    10441 ccatagtatt aatagaatat aggaaaatat taagacaaag aaaaatagac aggttaatta
    10501 aaagaataag taaaagagca gaagacagtg gcaatgagag tgaaggagat caggaagaat
    10561 tatcagcact tgtggagatg gggcaccatg ctctttggga tattgatgat ctatagtgct
    10621 gcagggaatt tgtgggtcac agtatattat ggggtacctg tatggaaaga agcaaccacc
    10681 actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa tgtttgggcc
    10741 acacatgcct gtatacccac agaccccaac ccacaagaga tagtattaga aaatgtgaca
    10801 gaagatttta acatgtggaa aaataacatg gtagaacaga tgcaggagga tataatcagt
    10861 ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt tactctaaat
    10921 tgcactgatg agttgaagaa tgctactaat accactagct agtactaata cccctagtgg
    10981 tagctggaaa aagatggaaa gaggagaaat aaagaactgc tctttcaatg tcttaggaga
    11041 taagaagcag aaagcatatg cactctttta taaacttgat gtagtaccaa tagataatga
    11101 taagaatagt accaaatata ggttgataag ttgtaacacc tcagtcacta cacaggcctg
    11161 tccaaagata tcctttgagc caattcccat acattattgt gccccggctg gttttgcgat
    11221 tctaaagtgt aaagataaga agttcaatgg aacaggatca tgtaaaaatg tcagcacagt
    11281 acaatgtaca catggaatta agccagtagt atcaactcaa ctgctgttaa atggcagcct
    11341 agcagaagaa gaggtagtaa ttagatctgc caatttctcg gacaatgcta aaaccataat
    11401 agtacagctg aaagaagctg tagaaattaa ttgcacaaga cccaacaaaa aaacaagaaa
    11461 acgtataact acgggaccag ggagagtata ttatacaaca ggagaaatag taggagatat
    11521 aagacaagca cattgtaacc ttagtagagc aaaatggaat gacactttaa gacagatagt
    11581 tataaaatta agagaacaat ttgagaataa aacaatagtc tttaatcaat cctcaggagg
    11641 ggacccagaa attgtaatgc atagttttaa ttgtggaggg gaatttttct actgtaagtc
    11701 aacacaactg tttaatagta cttgggctgg gaataatact tggaatagta gtgctgaaag
    11761 gtcagatgac actggaggaa atatcacact cccatgcaga ataaaacaaa ttataaacat
    11821 gtggcaggaa gtaggaaaag caatgtatgc ccctcccatc agcggacaaa ttagatgtac
    11881 atcaaatatt acagggctgc tattaacaag agatggtggt acgagcgata ccaataccac
    11941 cgagaccttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    12001 atataaagta gtaaaaattg aaccattagg agtagcaccc accacggcaa agagaagagt
    12061 ggtgcaaaga gaaaaaagag cagtgggaat aggagctctg ttccttgggt tcttgggagc
    12121 agcaggaagc actatgggcg cagcgtcaat gacgttgacg gtacaggcca gacaattatt
    12181 gtctggtata gtgcaacagc agaacaattt gctgagggct attgaggcgc aacagcatct
    12241 gttgcaactc acagtctggg gcatcaagca gctccaggca agagtcctgg ctgtggaaag
    12301 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    12361 cactgctgtg ccttggaatg ctagttggag taataaatct ctgaatcaga tttgggataa
    12421 catgacttgg atgcagtggg aaagagaaat tgaaaattac acagacttaa tatacaactt
    12481 aattgaagaa tcgcagaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    12541 atgggcaagt ttgtggaatt ggtttacaat aacaaactgg ctgtggtata taaaaatatt
    12601 cataatgata gtaggaggct tgataggttt aagaatagtt tttactgtac tttctatagt
    12661 gaatagagtt aggcagggat actcaccatt gtcgtttcag acccacctcc caaccccgag
    12721 gggacccgac aggcccgaag gaatcgaaga agaaggtgga gagagagaca gagacagatc
    12781 cggtcgatta gtgaacggat tcttagcact tttctgggac gatctgcgga gcctgtgcct
    12841 cttcagctac caccgcttga gagacttaat cttggttgta acgaggattg tggaacttct
    12901 gggacgcagg gggtgggaag ccctcaagta ttggtggagt ctcctacagt attggagcca
    12961 ggaactaaag aatagtgctg ttaacttgct taatgtcaca gccatagcag tagctgaggg
    13021 aacagatagg gttatagaag tagtacaaag aacttataga gctattctcc acatacctag
    13081 aagaataaga cagggcttgg aaaggctttt gctataagat gggtggcaag tggtcaaaac
    13141 gtatggaggg tggatggcat gctgtaaggg aaagaatgag acgagctgag ccagcagcag
    13201 atggggtggg agcagtatct agagacctgg aaaaacatgg agcaatcaca agtagcaata
    13261 cagcagctaa caatgctgat tgtgcctggc tggaagcaca agaggaggag gaggtgggtt
    13321 ttccagtcag acctcaggta cctttaagac caataactta caaggcagct gtagatatta
    13381 gccacttttt aaaagaaaag gggggactgg aagggctaat tttctcccaa agaagacaag
    13441 atatccttga tctgtggacc taccacacac aaggctactt ccctgattgg cagaactaca
    13501 caccagggcc agggatcaga tatccactga cctttggatg gtgtttcaag ctagtaccag
    13561 ttgatccaga gaagatagaa gaggccaatg aaggagaaaa caacagcttg ctacacccta
    13621 tgagccagca tgggatggat gacccggaga gagaagtgct agagtggagg tttgacagcc
    13681 gcctagcatt tcatcacatg gcccgagagc tgcatccgga gtactacaag aaccgatgac
    13741 atcgcgcttt ctacaaggga ctttccgctg gggactttcc aggggaggcg tggcctgggc
    13801 gggaccgggg agtggcgagc cctcagatgc tgcatataag cagctgcttt tgcctgtact
    13861 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctagct agggaaccca
    13921 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    13981 tgtgactctg gtagctagag atccctcata cccttttagt cagtgtggaa aatctctagc
    14041 aaaaattcct agctgcctag aaataaaaca ctatgctttt gaataacata tgacttaaaa
    14101 gaagaattta tgatggctat tagaaaatat tttaaactga ttgaaaatga aggtgaacta
    14161 caatgatcta gattgctgct gctgaaatgc taagatgaaa tttatagcct taagtctatg
    14221 cattggaaaa gaaagctttt ttaaggaggt agaaaaggaa taggaaatca aacctaaaga
    14281 cagtagaagt aaagaaataa tgaagacaag agcagaaatt agtgaataga aagtaatctt
    14341 acatagagaa aattgacaaa gctaagaaaa agttggttct ttgaaaagat taatgtaatt
    14401 gaccacttag caaaactgat aaaaggagga tggggagggg gagagagaac ataaatttaa
    14461 ataataggaa ttgaaaaatg tcacatcact acggatcctc tagagtcgac ctgcaggcat
    14521 gcaagctttc cctatagtga gtcgtattag agcttggcgt aatcatggtc atagctgttt
    14581 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    14641 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    14701 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    14761 gggagaggcg gtttgcgtat tgggcgct
//
