LOCUS       sequence-288042-       10690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SARS-CoV-2 S (spike) protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10690)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 10690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pTwist-EF1alpha-SARS-CoV-2-S-2xStrep
FEATURES             Location/Qualifiers
     source          1..10690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3847..3870
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3907..3930
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    3999..4120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4010..4029)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4064..4083
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4235..4281
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4311..4447
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4492..4563
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(4789..5377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4869..4888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5877..7086
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6923..6942
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7090..7689
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7090..7109)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7586..7606
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7716..7764
                     /note="synthetic polyadenylation signal"
     CDS             join(8030..8098,8099..8890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8248..8267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8903..9039
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9191..9262
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9267..9313)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9331..9379
                     /note="synthetic polyadenylation signal"
     promoter        9416..10594
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9647..10585
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10542..10562
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gccgccacca tgtttgtttt cttggttctt ttgccactgg taagcagtca gtgcgtcaac
       61 ctgaccaccc ggacacaact cccacccgca tacacgaaca gtttcactag gggggtttac
      121 taccctgaca aagtttttag atctagtgtt ttgcattcca cgcaagatct gttccttccg
      181 ttcttttcaa atgtcacgtg gttccatgct attcacgtca gcggcactaa tggaacgaaa
      241 cgattcgata accccgtctt gcccttcaac gatggcgtat atttcgcgtc aacagagaaa
      301 agtaatataa ttagaggctg gatctttggt acgactctcg acagtaagac gcagtcactt
      361 ctgattgtaa acaatgcaac taatgtagtc atcaaggtgt gtgagttcca gttctgcaat
      421 gatcctttct tgggagtgta ttatcataag aataacaaat cctggatgga gtctgagttc
      481 cgggtatatt cttcagcaaa caattgcacg tttgaatatg taagccaacc tttccttatg
      541 gacctggaag ggaagcaagg taatttcaag aatcttaggg aatttgtgtt taaaaatata
      601 gatggctact tcaagatcta ctcaaagcat accccgatta acctcgtcag agacctgccc
      661 caagggttca gtgcgcttga acccctggta gatttgccaa tcggcataaa tataaccagg
      721 ttccagacac tgctcgccct ccaccggagc taccttactc caggagattc atctagcggg
      781 tggacagctg gagcggcagc ttactacgtc ggttaccttc aaccgaggac attcttgctt
      841 aaatacaatg agaatggcac gataactgac gccgtagatt gcgcgcttga tccgctgtca
      901 gaaacgaagt gtacacttaa aagttttacg gtcgaaaagg gaatctacca aacatcaaat
      961 tttagggtac aacctacgga atctatcgta cgcttcccga atatcactaa cttgtgtccg
     1021 ttcggcgagg tttttaatgc gaccaggttt gcttccgtgt acgcctggaa caggaaacgg
     1081 atctccaatt gtgtcgccga ttactccgtc ttgtataatt cagcatcttt cagcacgttt
     1141 aaatgttacg gagtttcccc cacaaaattg aatgaccttt gctttacgaa cgtctacgcg
     1201 gattcatttg taatccgggg ggacgaagtt aggcaaattg cgccagggca gactggcaag
     1261 atagctgact ataattataa attgccggat gactttacgg gctgtgtgat tgcttggaac
     1321 tcaaataatc tggactcaaa ggtaggggga aattataact acctttacag gctgttccgg
     1381 aagagtaatc tgaagccatt cgaaagagat ataagtacag agatctacca agctggaagc
     1441 accccctgca atggtgttga aggattcaat tgttatttcc cattgcaatc ctatggtttt
     1501 caaccgacga atggggtggg ataccaacca tatcgagttg tggttctcag tttcgagttg
     1561 cttcatgctc ctgcgacagt atgtggacca aaaaaatcta ctaatctggt gaagaataaa
     1621 tgcgtcaatt ttaattttaa tggtctgaca ggtaccggag tgcttacgga atctaacaag
     1681 aagtttcttc cttttcaaca gttcgggagg gacatagccg acacaactga cgcggtaaga
     1741 gatccgcaga cgttggaaat tctcgatata actccatgct catttggagg agtgagtgtt
     1801 atcactcctg gtacgaacac cagtaatcag gttgcagttc tttatcagga cgtgaattgt
     1861 acagaggtcc cggttgcgat acacgcggac cagctgacgc ccacgtggcg ggtttactca
     1921 actgggtcaa atgtgtttca aaccagagcc ggctgcctca taggagccga gcatgtgaac
     1981 aacagttatg aatgtgacat acctatcggg gcggggatct gcgcttcata tcaaacgcaa
     2041 accaattccc cgcgaagagc gcgaagtgtt gcaagtcaaa gtattatagc gtatacgatg
     2101 agcttgggtg ctgagaactc cgtggcatat tcaaataatt ccatcgcaat tcccacgaat
     2161 tttacgattt ctgtgacgac ggagatattg ccggtttcaa tgactaagac gagtgttgac
     2221 tgtacgatgt acatctgcgg cgactcaacg gagtgttcca acctgctcct gcagtacggc
     2281 tcattctgca cccaactgaa tcgggcactt acgggtattg ctgttgaaca agacaaaaat
     2341 acgcaagagg tgttcgccca ggtcaagcag atctacaaga caccaccgat taaggacttt
     2401 ggggggttta atttttctca gatacttccc gatcccagta aacctagtaa aaggtcattt
     2461 atagaggacc tcttgttcaa caaggtaacg ctcgctgatg caggattcat caagcagtat
     2521 ggcgattgtc ttggggacat tgcggcgcgc gacctgattt gtgcccagaa gttcaacgga
     2581 ctgacggtac ttccgcctct cctgaccgac gaaatgatcg cacaatacac ttctgcattg
     2641 cttgctggca ccataacctc aggttggacc ttcggtgccg gggcggcctt gcaaatcccc
     2701 tttgctatgc aaatggcata tcgattcaat gggataggag ttactcaaaa tgttctgtat
     2761 gaaaaccaaa agctgatagc caatcaattt aatagtgcta ttgggaaaat tcaggacagc
     2821 ctgagtagta cggcttccgc tctcggcaaa ctccaagatg tcgttaacca aaatgcacaa
     2881 gctctcaata cacttgttaa gcaactgagc tccaacttcg gcgctatatc atcagttctt
     2941 aacgatattc tttcccgact cgacaaggtc gaagccgaag tccaaataga caggctgata
     3001 accgggaggc tgcaaagtct ccaaacgtat gttacccaac aactcatacg agcggccgag
     3061 attagggcgt cagccaacct cgccgctact aaaatgtctg aatgtgtact cgggcagtcc
     3121 aagcgcgtgg acttttgtgg caagggttat catctgatga gtttcccgca atcagcgcct
     3181 cacggagtgg tcttcctcca cgtgacgtat gtccctgcgc aagaaaaaaa ctttacgaca
     3241 gcccctgcca tttgtcatga cgggaaggcg cactttccac gcgaaggcgt ttttgtatcc
     3301 aacggcaccc actggtttgt cacacagcga aatttttatg agcctcagat cataactact
     3361 gacaatactt tcgtctcagg gaactgcgat gtagtcatag ggatcgtaaa caatacggta
     3421 tacgatcccc tccagcctga actggactca tttaaagagg agctggacaa gtatttcaaa
     3481 aatcacacat caccggacgt ggacctcgga gatattagtg gtattaacgc aagcgtagta
     3541 aatattcaaa aggaaatcga ccgcttgaat gaagtcgcga aaaacctgaa cgagtctctt
     3601 attgatcttc aagaactcgg taagtatgaa caatacatca agtggccttg gtacatatgg
     3661 cttggattca ttgcggggtt gatcgcaatc gtaatggtta ccattatgtt gtgttgcatg
     3721 acaagctgtt gcagctgcct caaaggttgt tgttcttgcg gtagttgctg caagttcgac
     3781 gaagatgatt ctgaacctgt tcttaaaggc gtaaaattgc actacacgct cgaaggcggc
     3841 gggggatgga gccatccaca attcgagaaa ggcggtggtt caggaggagg tagcgggggt
     3901 ggatcatggt cacatccgca gtttgaaaag taaggatccg caggcctctg ctagcttgac
     3961 tgactgagat acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg
     4021 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4081 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4141 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4201 caaatgtggt attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa
     4261 cgggtcttga ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc
     4321 agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc
     4381 aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta
     4441 tccttaaggt cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt
     4501 aatggtcttt tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc
     4561 ctaatctcat tggttacctt gggctatcga aacttaatta accagtcaag tcagctactt
     4621 ggcgagatcg acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc
     4681 tggtccttgc tattgcaccc gttctccgat tacgagtttc atttaaatca tgtgagcaaa
     4741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4981 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5221 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5341 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5401 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5461 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5521 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5581 agcgatctgt ctatttcgtt catccatagt tgcatttaaa tttccgaact ctccaaggcc
     5641 ctcgtcggaa aatcttcaaa cctttcgtcc gatccatctt gcaggctacc tctcgaacga
     5701 actatcgcaa gtctcttggc cggccttgcg ccttggctat tgcttggcag cgcctatcgc
     5761 caggtattac tccaatcccg aatatccgag atcgggatca cccgagagaa gttcaaccta
     5821 catcctcaat cccgatctat ccgagatccg aggaatatcg aaatcggggc gcgcctggcc
     5881 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc gtcacggcga gcgctgccac
     5941 gtcagacgaa gggcgcagga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     6001 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     6061 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     6121 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     6181 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     6241 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg
     6301 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     6361 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     6421 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     6481 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag cccttcgcta
     6541 atgcgggaaa gctcttattc gggtgagatg ggctgggcac catctgggga ccctgacgtg
     6601 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     6661 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     6721 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     6781 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     6841 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     6901 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     6961 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     7021 aattttcagt gttagacttg taaattgtcc gctaaattct ggccgttttt ggcttttttg
     7081 ttagacaaca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     7141 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7201 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7261 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7321 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7381 ttgagcggtt cccggctggc cgcgcagcaa cagatggagg gcctcctggc gccgcaccgg
     7441 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7501 ggtctgggca gcgccgtcgt gctccccgga gtggaggccg ccgagcgcgc cggggtgccc
     7561 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7621 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7681 ggtgcctagg ctagtatgta agcctagtct tagataataa aatcgctatc catcgaagat
     7741 ggatgtgtgt tggttttttg tgtgtgtaac gctaggcgcg cctggtgtac cgagaacgat
     7801 cctctcagtg cgagtctcga cgatccatat cgttgcttgg cagtcagcca gtcggaatcc
     7861 agcttgggac ccaggaagtc caatcgtcag atattgtact caagcctggt cacggcagcg
     7921 taccgatctg tttaaaccta gatattgata gtctgatcgg tcaacgtata atcgagtcct
     7981 agcttttgca aacatctatc aagagacagg atcagcagga ggctttcgca tgagtattca
     8041 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8101 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcgc gagtgggtta
     8161 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgctt
     8221 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8281 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc
     8341 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8401 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa
     8461 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8521 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8581 ggcaacaacc ttgcgtaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8641 gttgatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8881 gcattggtaa ccgattctag gtgcattggc gcagaaaaaa atgcctgatg cgacgctgcg
     8941 cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca
     9001 cttccatacg tgtcctcctt accagaaatt tatccttaag atcccgaatc gtttaaactc
     9061 gactctggct ctatcgaatc tccgtcgttt cgagcttacg cgaacagccg tggcgctcat
     9121 ttgctcgtcg ggcatcgaat ctcgtcagct atcgtcagct tacctttttg gcagcgatcg
     9181 cggctcccga catcttggac cattagctcc acaggtatct tcttccctct agtggtcata
     9241 acagcagctt cagctacctc tcaattcaaa aaacccctca agacccgttt agaggcccca
     9301 aggggttatg ctatcaatcg ttgcgttaca cacacaaaaa accaacacac atccatcttc
     9361 gatggatagc gattttatta tctaactgct gatcgagtgt agccagatct cgtgaggctc
     9421 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     9481 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     9541 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcactagt
     9601 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     9661 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     9721 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     9781 ttcgtggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgtgg cctggcctgg
     9841 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     9901 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     9961 tagtcttgta aatgcgggcc aagatcagca cactggtatt tcggtttttg gggccgcggg
    10021 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
    10081 gccaccgaga atcggacggg ggtagtctca agctgcccgg cctgctctgg tgcctggcct
    10141 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
    10201 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agcacaaaat ggaggacgcg
    10261 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
    10321 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
    10381 ctccagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
    10441 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
    10501 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
    10561 tcaaagtttt tttcttccat ttcaggtgtc gtgaaaacta cccctaaaag ccaaaagatc
    10621 tttgtcgatc ctaccatcca ctcgacacac ccgccagcgg ccgctgccaa gcttccgagc
    10681 tctcgaattc
//
