LOCUS       sequence-288043-        3913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP1
FEATURES             Location/Qualifiers
     source          1..3913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(55..82)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(174..260)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    313..412
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(956..1055)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1167..1186)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1245..1264)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1838..2371)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2560..2565,2566..2582,2583..2588))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3245..3833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3734..3753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
       61 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
      121 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
      181 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
      241 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
      301 gatctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      361 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagttgg catggagagc
      421 ctggtgcctg gcttcaacga aaagacccac gtgcagctgt ctctgccagt gctgcaggtg
      481 cgcgacgtgc tggtgagggg attcggtgac tctgtggagg aagtgctgtc tgaagctaga
      541 cagcacctga aggatggtac ctgcggactg gtggaggtgg aaaagggcgt gctgccccag
      601 ctggagcagc cttacgtgtt catcaagcgc agcgatgcta ggactgctcc ccacggacac
      661 gtgatggtgg aactggtggc cgagctggaa ggtatccagt acggtcgctc cggagagacc
      721 ctgggtgtgc tggtgccaca cgtgggtgaa atccccgtgg cttacagaaa ggtgctgctg
      781 cgtaagaacg gaaacaaggg tgccggcgga cactcctacg gtgctgacct gaagtctttc
      841 gacctgggcg acgagctggg aactgaccct tacgaggact tccaggaaaa ctggaacacc
      901 aagcacagct ccggagtgac cagagagctg atgcgtgaac tgaacggtgg ctaataccca
      961 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1021 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc tctggcccgt
     1081 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1141 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     1201 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     1261 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     1321 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1381 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     1441 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     1501 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     1561 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     1621 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     1681 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     1741 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     1801 attaggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
     1861 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     1921 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     1981 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     2041 gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga
     2101 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     2161 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     2221 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     2281 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     2341 gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     2401 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
     2461 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     2521 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
     2581 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
     2641 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
     2701 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
     2761 gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt gccggtggtg
     2821 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
     2881 gcccaggact ctagctatag ttctagtggt tggctactaa taggttgtat tgatgttgga
     2941 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3001 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3061 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     3121 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     3181 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3601 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3661 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3721 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3781 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3841 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3901 ctgcgttatc ccc
//
