LOCUS       sequence-288044-        9211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP3
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(56..83)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(175..261)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    314..413
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6255..6354)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6466..6485)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6544..6563)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(7137..7670)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7859..7864,7865..7881,7882..7887))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      8544..9132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9033..9052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgattctgt ggataaccgt attaccgcta gccaggaaga gtttgtagaa acgcaaaaag
       61 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
      121 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
      181 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
      241 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
      301 ggatctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
      361 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatggcccc
      421 taccaaggtg accttcggag acgacaccgt gatcgaggtg cagggttaca agagcgtgaa
      481 catcaccttc gagctggacg aacgcatcga caaggtgctg aacgaaaagt gctccgctta
      541 caccgtggag ctgggcaccg aggtgaacga attcgcctgc gtggtggccg acgctgtgat
      601 caagaccctg cagccagtgt ccgaactgct gacccctctg ggtatcgacc tggacgagtg
      661 gtctatggct acctactacc tgttcgacga gagcggcgaa ttcaagctgg cctctcacat
      721 gtactgcagc ttctaccctc ccgacgaaga cgaggaagag ggtgactgcg aagaggaaga
      781 gttcgagcct tccactcagt acgagtacgg caccgaagac gactaccagg gcaagcctct
      841 ggaattcgga gccacctctg ccgctctgca gcctgaagag gaacaggagg aagactggct
      901 ggacgacgac tcccagcaga ccgtgggtca gcaggacggc tctgaagaca accagaccac
      961 caccatccag accatcgtgg aggtgcagcc tcagctggag atggaactga ccccagtggt
     1021 gcagaccatc gaggtgaaca gcttctccgg ctacctgaag ctgaccgaca acgtgtacat
     1081 caagaacgct gacatcgtgg aggaagccaa gaaggtgaaa ccaaccgtgg tggtgaacgc
     1141 cgctaacgtg tacctgaagc acggcggagg tgtggctgga gctctgaaca aggctaccaa
     1201 caacgccatg caggtggaat ccgacgacta catcgctacc aacggtcctc tgaaggtggg
     1261 cggatcctgc gtgctgtctg gccacaacct ggccaagcac tgcctgcacg tggtgggacc
     1321 aaacgtgaac aagggagagg acatccagct gctgaagagc gcctacgaaa acttcaacca
     1381 gcacgaggtg ctgctggctc cactgctgtc tgccggaatc ttcggtgctg atcccatcca
     1441 cagcctgcgc gtgtgcgtgg acaccgtgag gaccaacgtg tacctggccg tgttcgacaa
     1501 gaacctgtac gacaagctgg tgagctcctt cctggagatg aagtccgaaa agcaggtgga
     1561 gcagaagatc gccgagatcc ccaaggagga agtgaagcct ttcatcaccg aatctaagcc
     1621 cagcgtggag cagagaaagc aggacgacaa gaagatcaag gcctgcgtgg aggaagtgac
     1681 caccaccctg gaggaaacca agttcctgac cgagaacctg ctgctgtaca tcgacatcaa
     1741 cggcaacctg caccctgact ccgctaccct ggtgtctgac atcgacatca ccttcctgaa
     1801 gaaggacgcc ccctacatcg tgggcgacgt ggtgcaggaa ggagtgctga ccgctgtggt
     1861 catccctacc aagaaggccg gtggcaccac cgagatgctg gccaaggctc tgcgtaaggt
     1921 gccaaccgac aactacatca ccacctaccc cggtcagggc ctgaacggtt acaccgtgga
     1981 ggaagctaag accgtgctga agaagtgcaa gtctgccttc tacatcctgc cctccatcat
     2041 ctctaacgag aagcaggaaa tcctgggcac cgtgagctgg aacctgcgcg aaatgctggc
     2101 ccacgctgag gaaaccagga agctgatgcc cgtgtgcgtg gagaccaagg ccatcgtgag
     2161 caccatccag agaaagtaca agggcatcaa gatccaggag ggtgtggtgg actacggcgc
     2221 tcgtttctac ttctacacct ccaagaccac cgtggcctct ctgatcaaca ccctgaacga
     2281 cctgaacgag accctggtga ccatgccact gggatacgtg acccacggtc tgaacctgga
     2341 ggaagccgct agatacatgc gttccctgaa ggtgcctgct accgtgagcg tgagcagccc
     2401 tgacgctgtg accgcctaca acggatacct gacctcctct agcaagaccc ctgaggaaca
     2461 cttcatcgaa accatcagcc tggccggttc ctacaaggac tggtcttaca gcggacagtc
     2521 cacccagctg ggcatcgagt tcctgaagcg cggagacaag agcgtgtact acacctccaa
     2581 cccaaccacc ttccacctgg acggtgaagt gatcaccttc gacaacctga agaccctgct
     2641 gtctctgcgc gaggtgagga ccatcaaggt gttcaccacc gtggacaaca tcaacctgca
     2701 cacccaggtg gtggacatga gcatgaccta cggacagcag ttcggtccta cctacctgga
     2761 cggagctgac gtgaccaaga tcaagccaca caactcccac gagggcaaga ccttctacgt
     2821 gctgcctaac gatgacaccc tgagagtgga ggctttcgaa tactaccaca ccaccgaccc
     2881 atccttcctg ggcaggtaca tgtctgccct gaaccacacc aagaagtgga agtaccccca
     2941 ggtgaacgga ctgaccagca tcaagtgggc cgacaacaac tgctacctgg ccaccgctct
     3001 gctgaccctg cagcagatcg aactgaagtt caacccaccc gctctgcagg acgcttacta
     3061 cagagcccgt gctggcgagg ccgctaactt ctgcgctctg atcctggcct actgcaacaa
     3121 gaccgtgggc gaactgggag acgtgagaga gaccatgagc tacctgttcc agcacgccaa
     3181 cctggactcc tgcaagcgtg tgctgaacgt ggtgtgcaag acctgcggcc agcagcagac
     3241 caccctgaag ggagtggaag ctgtgatgta catgggtacc ctgtcctacg agcagttcaa
     3301 gaagggcgtg cagatccctt gcacctgcgg aaagcaggcc accaagtacc tggtgcagca
     3361 ggaaagccca ttcgtgatga tgtccgctcc tccagcccag tacgaactga agcacggcac
     3421 cttcacctgc gcttccgagt acaccggcaa ctaccagtgc ggacactaca agcacatcac
     3481 ctctaaggag accctgtact gcatcgacgg agccctgctg accaagtcct ctgaatacaa
     3541 gggtcccatc accgacgtgt tctacaagga gaacagctac accaccacca tcaagcccgt
     3601 gacctacaag ctggacggcg tggtgtgcac cgaaatcgac cctaagctgg acaactacta
     3661 caagaaggac aactcttact tcaccgagca gcctatcgac ctggtgccaa accagcccta
     3721 ccctaacgcc agcttcgaca acttcaagtt cgtgtgcgac aacatcaagt tcgctgacga
     3781 cctgaaccag ctgaccggat acaagaagcc tgcctctaga gagctgaagg tgaccttctt
     3841 cccagacctg aacggcgacg tggtggctat cgactacaag cactacaccc caagcttcaa
     3901 gaagggagcc aagctgctgc acaagcccat cgtgtggcac gtgaacaacg ctaccaacaa
     3961 ggccacctac aagcctaaca cctggtgcat ccgttgcctg tggtctacca agccagtgga
     4021 aacctccaac tctttcgacg tgctgaagag cgaggacgct cagggcatgg acaacctggc
     4081 ctgcgaagac ctgaagcccg tgagcgagga agtggtggag aacccaacca tccagaagga
     4141 cgtgctggaa tgcaacgtga agaccaccga ggtggtggga gacatcatcc tgaagccagc
     4201 taacaacagc ctgaagatca ccgaggaagt gggtcacacc gacctgatgg ccgcttacgt
     4261 ggacaacagc tccctgacca tcaagaagcc caacgagctg agcagggtgc tgggtctgaa
     4321 gaccctggct actcacggtc tggctgctgt gaactctgtg ccatgggaca ccatcgctaa
     4381 ctacgccaag cctttcctga acaaggtggt gtccaccacc accaacatcg tgaccagatg
     4441 tctgaaccgc gtgtgcacca actacatgcc ctacttcttc accctgctgc tgcagctgtg
     4501 caccttcacc agaagcacca actcccgtat caaggctagc atgcctacca ccatcgccaa
     4561 gaacaccgtg aagagcgtgg gaaagttctg cctggaggct tccttcaact acctgaagtc
     4621 tccaaacttc agcaagctga tcaacatcat catctggttc ctgctgctct ccgtgtgcct
     4681 gggttccctg atctactcta ccgccgctct gggagtgctg atgtctaacc tgggtatgcc
     4741 tagctactgc actggttaca gagagggata cctgaacagc accaacgtga ccatcgccac
     4801 ctactgcacc ggcagcatcc catgctccgt gtgcctgtct ggactggaca gcctggacac
     4861 ctacccctcc ctggaaacca tccagatcac catctctagc ttcaagtggg acctgaccgc
     4921 cttcggtctg gtggctgagt ggttcctggc ctacatcctg ttcaccaggt tcttctacgt
     4981 gctgggcctg gccgctatca tgcagctgtt cttctcctac ttcgctgtgc acttcatcag
     5041 caactcctgg ctgatgtggc tgatcatcaa cctggtgcag atggctccca tctctgccat
     5101 ggtgcgcatg tacatcttct tcgccagctt ctactacgtg tggaagtcct acgtgcacgt
     5161 ggtggacggt tgcaactcct ctacctgcat gatgtgctac aagcgcaaca gggctaccag
     5221 ggtggagtgc accaccatcg tgaacggcgt gcgcaggtcc ttctacgtgt acgccaacgg
     5281 cggcaaggga ttctgcaagc tgcacaactg gaactgcgtg aactgcgaca ccttctgcgc
     5341 tggctccacc ttcatctctg acgaggtggc cagagacctg agcctgcagt tcaagcgtcc
     5401 aatcaacccc accgaccaga gctcctacat cgtggactct gtgaccgtga agaacggtag
     5461 catccacctg tacttcgaca aggctggcca gaagacctac gagcgccact ctctgagcca
     5521 cttcgtgaac ctggacaacc tgagggccaa caacaccaag ggcagcctgc ctatcaacgt
     5581 gatcgtgttc gacggaaagt ccaagtgcga ggaatctagc gccaagtccg ccagcgtgta
     5641 ctacagccag ctgatgtgcc agccaatcct gctgctggac caggctctgg tgtctgacgt
     5701 gggagacagc gccgaagtgg ccgtgaagat gttcgacgcc tacgtgaaca ccttctcctc
     5761 taccttcaac gtgcctatgg agaagctgaa gaccctggtg gctactgctg aggctgaact
     5821 ggctaagaac gtgtccctgg acaacgtgct gagcaccttc atctccgccg ctaggcaggg
     5881 tttcgtggac tctgacgtgg aaaccaaaga cgtggtggag tgcctgaagc tgtctcacca
     5941 gagcgacatc gaagtgaccg gcgactcctg caacaactac atgctgacct acaacaaggt
     6001 ggagaacatg accccaagag acctgggagc ttgcatcgac tgctccgccc gtcacatcaa
     6061 cgctcaggtg gccaagtctc acaacatcgc cctgatctgg aacgtgaagg acttcatgag
     6121 cctgtccgag cagctgcgca agcagatcag gagcgccgct aagaagaaca acctgccctt
     6181 caagctgacc tgcgctacca ccagacaggt ggtgaacgtg gtgaccacca agatcgccct
     6241 gaagggcgga taatacccaa ctttcttgta caaagttggc attataagaa agcattgctt
     6301 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     6361 agctgcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     6421 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     6481 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     6541 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     6601 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     6661 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     6721 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     6781 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     6841 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     6901 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     6961 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     7021 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     7081 ttatttttga cgaggggaaa ttaggccggg aagccgatct cggcttgaac gaattgttag
     7141 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     7201 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     7261 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     7321 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     7381 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     7441 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     7501 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     7561 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     7621 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     7681 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     7741 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     7801 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     7861 tttacgaacc gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt
     7921 gcgtcacccg gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa
     7981 cgagcgcaag gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta
     8041 cggcaaggtg ctgtgcacgg atctgccctg gcttcaggag atcggaagac ctcggccgtc
     8101 gcggcgcttg ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga
     8161 aggcgagcat cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctactaat
     8221 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     8281 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     8341 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     8401 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     8461 cgcaagctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9181 tttgctcaca tgttctttcc tgcgttatcc c
//
