LOCUS       sequence-288061-       10416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..10416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2734..2757
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2794..2817
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    join(2829..3403,3404..3406,3407..3415)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2996..3013)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3223..3242
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3435..4034
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3435..3454)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3931..3951
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4048..4636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4101..4121)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4519..4530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4843..5476
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5604..5620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5604..5620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5617..5639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5628..5644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5652..5658,5659..5676,5677..5682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5697..5718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5835..5852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6006..6594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6086..6105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6765..7556,7557..7625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7388..7407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7626..7730)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7778..7912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7815..7834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7869..7888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8080..8713
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8760..8885
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9382..9615
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9800..9844
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10107..10222
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        join(10416,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
     1201 ttcgccgcca ccatgaagat tgtaaacaat tggcttaaac aacttattaa agtaaccctg
     1261 gtattcttgt ttgtcgccgc aatcttttac ttgataacac cggtccacgt gatgtctaaa
     1321 cacacagact ttagtagtga gataatcgga tataaagcta tcgatggcgg cgttacccgc
     1381 gacatcgctt caaccgacac atgtttcgct aataagcacg ccgattttga cacttggttt
     1441 agtcaaagag gaggaagcta cacaaacgac aaggcttgtc cgcttatcgc ggctgtaata
     1501 acaagggaag ttgggtttgt cgtgcctggt ttgccaggta ctatactcag aaccacaaac
     1561 ggtgactttc tgcacttctt gccaagggtt tttagcgcgg taggcaatat ctgctatacg
     1621 ccaagcaagc tgattgaata caccgacttt gcgacgagcg catgtgtcct cgctgcagag
     1681 tgtacgatat ttaaggatgc gagcggaaag cccgtacctt actgctatga tactaatgtc
     1741 cttgagggat cagttgcgta tgagagtctt aggccggata cacgatatgt gctcatggat
     1801 ggttccataa tccagttccc aaatacctac ctcgaagggt ccgttcgggt cgtcaccaca
     1861 tttgatagcg aatattgcag acacggaaca tgcgaacgga gtgaagcagg ggtctgcgtc
     1921 tccacaagtg ggcggtgggt cctcaataac gattattacc ggagcctgcc cggcgtgttt
     1981 tgcggggtcg atgcggttaa cttgttgacg aatatgttta cgcctctcat acaacccatt
     2041 ggcgcactgg atatatctgc aagtatagtc gctggaggca ttgtggcaat cgtggttacg
     2101 tgtttggcgt attattttat gaggttccgc cgggctttcg gggaatattc ccacgtagtc
     2161 gccttcaaca cactcttgtt tctcatgtct ttcactgtac tgtgtttgac tcctgtctac
     2221 tctttcttgc cgggtgtata ctcagttatt tacttgtacc tcacgtttta tttgacaaac
     2281 gatgtttcat ttctcgcgca tatccaatgg atggtgatgt ttacgccatt ggtgcctttc
     2341 tggatcacga tcgcttatat catttgcatc agcaccaaac acttctattg gttctttagt
     2401 aactacttga agcgcagggt tgtttttaac ggtgtcagct tctcaacgtt tgaagaggca
     2461 gcactgtgta ctttccttct gaataaggaa atgtatctca agctgcggtc cgatgtcctg
     2521 cttccgctta cccagtataa tcgctatctc gccctgtata ataagtacaa gtacttctca
     2581 ggcgcgatgg atacaaccag ttatagagag gctgcctgct gccatcttgc caaagctttg
     2641 aatgacttca gcaatagtgg ctcagacgtt ctctaccaac caccgcaaac ttccataacc
     2701 tcagccgtac tccagctcga aggcggcggg ggatggagcc atccacaatt cgagaaaggc
     2761 ggtggttcag gaggaggtag cgggggtgga tcatggtcac atccgcagtt tgaaaagtaa
     2821 ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2881 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2941 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3001 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3061 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3121 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3181 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3241 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3301 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     3361 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa
     3421 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     3481 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     3541 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     3601 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     3661 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     3721 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     3781 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     3841 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     3901 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     3961 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     4021 agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     4081 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4141 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4201 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4261 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4321 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4381 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4441 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4501 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4561 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4621 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     4681 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     4741 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     4801 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     4861 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     4921 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     4981 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     5041 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     5101 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     5161 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     5221 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     5281 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5341 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5401 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5461 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5521 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     5581 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5641 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5701 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5761 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5821 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5881 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5941 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6181 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6421 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6541 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6601 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6661 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6721 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6781 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6841 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6901 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6961 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7021 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7081 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7141 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7201 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7261 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7321 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7381 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7441 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7501 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7561 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7621 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7681 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7741 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
     7801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     7921 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     7981 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     8041 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     8101 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     8161 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     8221 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     8281 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     8341 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     8401 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     8461 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     8521 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8581 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8641 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8701 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8761 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     8821 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8881 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8941 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     9001 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     9061 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     9121 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     9181 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     9241 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     9301 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     9361 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     9421 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     9481 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     9541 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     9601 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     9661 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     9721 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     9781 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     9841 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     9901 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     9961 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
    10021 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
    10081 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
    10141 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10201 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
    10261 gatgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt
    10321 aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct
    10381 ctcgtcatca ctgaggtgga gaagagcatg cgtgag
//
